Recruitment of Condensin to Replication Origin Regions by ParB/SpoOJ Promotes Chromosome Segregation in B. subtilis
Recruitment of Condensin to Replication Origin Regions by ParB/SpoOJ Promotes Chromosome Segregation in B. subtilis
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DOI：
10.1016/j.cell.2009.02.035
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发表时间：
2009-05-15
期刊：
影响因子：
64.5
通讯作者：
Errington, Jeff
中科院分区：
文献类型：
--
作者：
Gruber, Stephan;Errington, Jeff
DNA复制产物的正确分离在所有细胞中都是必不可少的。在枯草芽孢杆菌中，两种蛋白质复合物与此过程有关：ParAB同系物Soj和SpoOJ，以及细菌Smc/ScpAB复合物，也称为凝聚素。在这里，我们表明，Smc是高度富集在复制起点周围的区域，特别是附近的parS网站SpoOJ结合，并在高度转录的基因。此外，我们发现，有效的招聘Smc复制起点周围的一个大区域取决于SpoOJ的存在。我们发现，SpoOJ执行两个独立的功能：通过Soj启动DNA复制的调节和Smc招募促进染色体分离。我们的研究结果表明，两个广泛保守的系统参与染色体复制和分离之间的直接功能相互作用。
Proper segregation of DNA replication products is essential in all cells. In Bacillus subtilis, two protein complexes have been implicated in this process: the ParAB homologs, Soj and SpoOJ, and the bacterial Smc/ScpAB complex, also called condensin. Here we demonstrate that Smc is highly enriched in the region around the origin of replication, specifically near parS sites to which SpoOJ binds and at highly transcribed genes. Furthermore, we find that efficient recruitment of Smc to a large region around the origin of replication depends on the presence of SpoOJ. We show that SpoOJ performs two independent functions: regulation of initiation of DNA replication via Soj and promotion of chromosome segregation by Smc recruitment. Our results demonstrate a direct functional interaction between two widely conserved systems involved in chromosome replication and segregation.