Split DNA enzyme for visual single nucleotide polymorphism typing
Split DNA enzyme for visual single nucleotide polymorphism typing
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DOI：
10.1021/ja711192e
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发表时间：
2008-03-12
影响因子：
15
通讯作者：
Kolpashchikov, Dmitry M.
中科院分区：
文献类型：
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作者：
Kolpashchikov, Dmitry M.
据报道，一种探针可以在两种DNA序列中的一种存在下改变溶液的颜色，这两种DNA序列只相差一个核苷酸。探针由两个寡脱氧核糖核苷酸组成，当与互补分析物的邻近片段杂交时，形成类似过氧化氢酶的DNA酶。活性过氧化物酶催化无色底物氧化成有色产物。这种探针可以直观地检测出阿尔茨海默病相关DNA的突变。
A probe that changes solution color in the presence of only one of the two DNA sequences, which differ by a single nucleotide, is reported. The probe consists of two oligodeoxyribonucleotides, which form a hydrogen peroxidase-like DNA enzyme when hybridized to the abutting fragments of the complementary analyte. The active peroxidase catalyzes oxidation of colorless substrates to the colored products. The probe allows visual detection of a mutation in Alzheimer's disease related DNA.