Protein expression and subcellular localization of familial acute myelogenous leukemia-related factor
Protein expression and subcellular localization of familial acute myelogenous leukemia-related factor
复制标题
家族性急性髓性白血病相关因子的蛋白表达和亚细胞定位。
DOI：
10.3892/or.2013.2773
复制
发表时间：
2013-12-01
期刊：
影响因子：
4.2
通讯作者：
Wang, Shaoyuan
中科院分区：
文献类型：
--
作者：
Huang, Yuanmao;Chen, Lulu;Wang, Shaoyuan
本研究旨在评估家族性急性髓细胞性白血病相关因子（FAMLF）的表达和亚细胞分布。利用生物信息学方法鉴定了预测的FAMLF基因开放阅读框架的14个氨基酸的表位。这种多肽被合成，与钥孔血蓝蛋白缀合，随后用于产生抗体。抗体效价和特异性分别使用ELISA和Western印迹测定进行表征。该抗体检测到FAMLF蛋白在几种人白血病细胞系、来自一名急性髓性白血病患者和一名慢性髓性白血病患者的骨髓细胞中的表达，但在健康受试者的骨髓细胞中未检测到。FAMLF/GFP融合蛋白在NIH 3 T3细胞核和胞浆中均有表达。我们的研究结果表明，FAMLF基因在AML患者中表达，但在健康对照组中不表达，表明其与AML相关。
The present study was designed to evaluate the expression and subcellular distribution of the familial acute myelogenous leukemia-related factor (FAMLF). A 14-amino acid epitope of the predicted open reading frame of the FAMLF gene was identified using bioinformatics. This polypeptide was synthesized, conjugated to keyhole limpet hemocyanin and was subsequently used to produce antibodies. The antibody titer and specificity were characterized using ELISA and western blot assays, respectively. The antibody detected FAMLF protein expression in several human leukemia cell lines, bone marrow cells derived from one acute myeloid leukemia patient and one chronic myeloid leukemia patient, but not in bone marrow cells of healthy subjects. The FAMLF/GFP fusion protein was expressed in both the nucleus and the cytoplasm of transfected NIH3T3 cells. Our results demonstrate that the FAMLF gene is expressed in an AML patient but not in healthy controls, suggesting its association with AML.