A single dual-emissive nanofluorophore test paper for highly sensitive colorimetry-based quantification of blood glucose
A single dual-emissive nanofluorophore test paper for highly sensitive colorimetry-based quantification of blood glucose
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单一双发射纳米荧光团试纸，用于基于比色法的高灵敏度血糖定量
DOI：
10.1016/j.bios.2016.07.021
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发表时间：
2016-12-15
影响因子：
12.6
通讯作者：
Zhang, Zhongping
中科院分区：
文献类型：
--
作者：
Huang, Xiaoyan;Zhou, Yujie;Zhang, Zhongping
荧光试纸在诊断、环境和食品检测中有着广泛的应用前景，但与传统的基于染料吸收的pH试纸不同，荧光试纸通常局限于通过荧光亮度进行定性是/否检测，基于比色的定量仍然是一项具有挑战性的任务。在这里，我们报告了一个单一的双发射纳米荧光探针，以实现连续的颜色变化，从蓝色到红色的血糖的定量测试文件。将红色量子点嵌入二氧化硅纳米颗粒中作为稳定的内标发射，并将蓝色碳量子点（CD）进一步共价连接到二氧化硅表面上，其中蓝色与红色的比例荧光强度被控制在5：1。当葡萄糖氧化诱导Fe ~（3+）离子形成时，Cd ~（3+）的蓝色发光被电子转移到Fe ~（3+）上而猝灭，并随着葡萄糖的加入呈现出一系列从蓝色到红色的连续颜色变化。用探针墨水打印的高质量试纸显示出剂量敏感的变色能力，人血清中5、7、9、11 mM葡萄糖（正常：3-8 mM）的差异明显。试纸测得的血糖值与标准血糖仪测得的血糖值基本一致。该方法为荧光试纸在生物检测中的广泛应用打开了一扇窗。(C)2016爱思唯尔B. V.保留所有权利。
Fluorescent test papers are promising for the wide applications in the assays of diagnosis, environments and foods, but unlike classical dye-absorption-based pH test paper, they are usually limited in the qualitative yes/no type of detection by fluorescent brightness, and the colorimetry-based quantification remains a challenging task. Here, we report a single dual-emissive nanofluorophore probe to achieve the consecutive color variations from blue to red for the quantification of blood glucose on its as-prepared test papers. Red quantum dots were embedded into silica nanoparticles as a stable internal standard emission, and blue carbon dots (CDs) were further covalently linked onto the surface of silica, in which the ratiometric fluorescence intensity of blue to red is controlled at 5:1. While the oxidation of glucose induced the formation of Fe3+ ions, the blue emission of CDs was thus quenched by the electron transfer from CDs to Feat, displaying a serial of consecutive color variations from blue to red with the dosage of glucose. The high-quality test papers printed by the probe ink exhibited a dosage-sensitive allochromatic capability with the clear differentiations of 5, 7, 9, 11 mM glucose in human serum (normal: 3-8 mM). The blood glucose determined by the test paper was almost in accordance with that measured by a standard glucometer. The method reported here opens a window to the wide applications of fluorescent test paper in biological assays. (C) 2016 Elsevier B.V. All rights reserved.