IDENTIFICATION OF EXTRACELLULAR CARBONIC-ANHYDRASE OF CHLAMYDOMONAS-REINHARDTII
IDENTIFICATION OF EXTRACELLULAR CARBONIC-ANHYDRASE OF CHLAMYDOMONAS-REINHARDTII
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DOI：
10.1104/pp.76.2.472
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发表时间：
1984-01-01
期刊：
影响因子：
7.4
通讯作者：
GROSSMAN, AR
中科院分区：
文献类型：
--
作者：
COLEMAN, JR;BERRY, JA;GROSSMAN, AR
研究了对莱茵梭菌碳酸氢酶活性的诱导作用，并鉴定了与该活性有关的多肽。这种多肽在5%的CO2中光自养生长时不能合成，但在低浓度的CO2(空气中的CO2)下可以诱导合成。在缺乏细胞壁的突变株CW-15中，空气适应细胞碳酸氢酶活性的80%-90%存在于生长介质中。如果用自溶素处理野生型细胞，80%-90%的碳酸氢酶被释放出来，自溶素是一种水解酶，负责在莱茵梭菌交配过程中降解细胞壁。这些数据扩展了金普尔等人的工作。(1983)，并表明碳酸酐酶的大部分位于周质空间或松散地结合在藻细胞壁上。与碳酸酐酶活性相关的多肽的分子量为.apprx。37000美元。有几条证据表明，这种多肽对碳酸酐酶活性有作用：它出现在莱茵梭菌从5%CO2生长转移到二氧化碳空气中生长的过程中；它位于周质空间或与细胞壁相关，就像大部分碳酸酐酶活性一样；它与丹磺酰胺结合，丹磺拉胺是一种酶抑制剂，在紫外光照射下会发出荧光；抑制碳酸酐酶活性的抗体只与这37,000道尔顿物种发生交叉反应。
The induction of carbonic anhydrase activity in C. reinhardtii was examined and the polypeptide responsible for this activity was identified. This polypeptide was not synthesized when the alga was grown photoautotrophically on 5% CO2, but its synthesis was induced under low concentrations of CO2 (air levels of CO2). In CW-15, a mutant of C. reinhardtii which lacks a cell wall, between 80-90% of the carbonic anhydrase activity of air-adapted cells was present in the growth medium. Between 80-90% of the carbonic anhydrase is released if wild type cells are treated with autolysin, a hydrolytic enzyme responsible for cell wall degradation during mating of C. reinhardtii. These data extend the work of Kimpel, et al. (1983), and indicate that the bulk of the carbonic anhydrase is located either in the periplasmic space or is loosely bound to the algal cell wall. The polypeptide associated with carbonic anhydrase activity has a MW of .apprx. 37,000. Several lines of evidence indicate that this polypeptide is responsible for carbonic anhydrase activity: it appears following the transfer of C. reinhardtii from growth on 5% CO2 to growth on air levels of CO2; it is located in the periplasmic space or associated with the cell wall, like the bulk of the carbonic anhydrase activity; it binds dansylamide, an inhibitor of the enzyme which fluoresces upon illumination with UV light; and antibodies which inhibit carbonic anhydrase activity only cross-react with this 37,000 dalton species.