Spatiotemporal expression of duplicate AGAMOUS orthologues during floral development in Phalaenopsis
Spatiotemporal expression of duplicate AGAMOUS orthologues during floral development in Phalaenopsis
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DOI：
10.1007/s00427-005-0057-0
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发表时间：
2006-02
影响因子：
2.4
通讯作者：
I. Song;Toru M. Nakamura;T. Fukuda;J. Yokoyama;Takuro Ito;H. Ichikawa;Y. Horikawa;T. Kameya;A. Kanno
中科院分区：
文献类型：
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作者：
I. Song;Toru M. Nakamura;T. Fukuda;J. Yokoyama;Takuro Ito;H. Ichikawa;Y. Horikawa;T. Kameya;A. Kanno
AGAMOUS（AG）家族MADS-box基因在控制显花植物生殖器官发育中起着重要作用。为了了解兰花花发育的分子机制，我们从蝴蝶兰中分离并鉴定了两个AG样基因，分别命名为PhalAG 1和PhalAG 2。系统发育分析表明，PhalAG 1和PhalAG 2分别属于C和D谱系，在AG基因家族中处于不同的系统发育位置。逆转录-聚合酶椅式反应（RT-PCR）分析表明，PhalAG 1和PhalAG 2在花芽中表达，而在营养器官中不表达。原位杂交结果表明，PhalAG 1和PhalAG 2基因在蝴蝶兰花发育过程中的唇、柱和胚珠中均有表达，但表达模式差异不大。这些结果表明，蝴蝶兰中的两个类AG基因在花发育过程中存在冗余作用。
TheAGAMOUS(AG) family of MADS-box genes plays important roles in controlling the development of the reproductive organs of flowering plants. To understand the molecular mechanisms behind the floral development in the orchid, we isolated and characterized twoAG-like genes fromPhalaenopsisthat we denotedPhalAG1andPhalAG2. Phylogenetic analysis indicated thatPhalAG1andPhalAG2fall into different phylogenetic positions in theAGgene family as they belong to the C- and D-lineages, respectively. Reverse transcription-polymerase chair reaction (RT-PCR) analyses showed thatPhalAG1andPhalAG2transcripts were detected in flower buds but not in vegetative organs. Moreover, in situ hybridization experiments revealed thatPhalAG1andPhalAG2hybridization signals were observed in the lip, column, and ovule during the floral development ofPhalaenopsis, with little difference between the expression patterns of the two genes. These results suggest that bothAG-like genes inPhalaenopsisact redundantly with each other in floral development.