Precise standardization of reagents for complement fixation.
Precise standardization of reagents for complement fixation.
复制标题
补体固定试剂的精确标准化。
DOI：
10.4269/ajtmh.1963.12.103
复制
发表时间：
1963
期刊：
影响因子：
--
通讯作者：
E. H. Fife
中科院分区：
文献类型：
--
作者：
J. F. Kent;E. H. Fife
总结
本文介绍了一种应用于传染病血清学诊断的精确补体结合试验。给出了溶血系统的标准化和控制的详细定量方法。补体固定被证明是受非抗体血清成分的浓度，以及由初级孵育的条件（时间和温度），和这些因素对抗原标准化的影响是说明与同源抗血清的反应的密螺旋体，锥虫和旋毛虫抗原的例子。
Summary
A precise complement-fixation test, applied diversely in the serodiagnosis of infectious disease, is described. Detailed quantitative methods for standardization and control of the hemolytic system are given. Complement fixation is shown to be affected by the concentration of nonantibody serum components, as well as by the conditions (time and temperature) of primary incubation, and the influence of these factors on antigen standardization is illustrated with examples from the reactions of treponemal, trypanosomal, and trichinal antigens with homologous antisera.