Mycobacterium pseudoshottsii sp nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)
Mycobacterium pseudoshottsii sp nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)
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DOI：
10.1099/ijs.0.63343-0
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发表时间：
2005-05-01
影响因子：
2.8
通讯作者：
Ottinger, CA
中科院分区：
文献类型：
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作者：
Rhodes, MW;Kator, H;Ottinger, CA
在一次分枝杆菌病流行期间，从切萨皮克条纹鲈（Morone saxatilis）体内分离到一组生长缓慢的产光显色分枝杆菌。生长特性、耐酸性和16 S rRNA基因测序结果与分枝杆菌属一致，生化反应、生长特性和分枝菌酸谱（HPLC）与肖氏分枝杆菌相似，肖氏分枝杆菌是一种在同一次动物流行中分离的无色素分枝杆菌。16 SrRNA基因、编码输出重复蛋白（erp）的基因和编码65 kDa热休克蛋白（hsp 65）的基因的测序和hsp 65基因的限制性内切酶分析表明这组分离物是独特的。通过PCR检测与溃疡分枝杆菌相关的插入序列IS 2404和IS 2606。这些分离株与其他生长缓慢的有色分枝杆菌的区别在于它们不能在37 ℃下生长，产生烟酸和尿素酶，缺乏硝酸盐还原酶，吐温80水解阴性，对异烟肼（1 μ g/ml）、对硝基苯甲酸、硫乙腙和噻吩-2-甲酰肼具有抗性。基于该多相研究，提出这些分离物代表一个新种，假肖氏分枝杆菌新种。模式菌株L15（T）已保藏在美国典型培养物保藏中心（ATCC BAA-883（T））和国家典型培养物保藏中心（UK）（NCTC 13318（T））。
A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass (Morone saxatilis) during an epizootic of mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus Mycobacterium, Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of Mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein (erp) and the gene encoding the 65 kDa heat-shock protein (hsp65) and restriction enzyme analysis of the hsp65 gene demonstrated that this group of isolates is unique. Insertion sequences associated with Mycobacterium ulcerans, IS2404 and IS2606, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 degrees C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 mu g ml(-1)), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, Mycobacterium pseudoshottsii sp. nov. The type strain, L15(T), has been deposited in the American Type Culture Collection as ATCC BAA-883(T) and the National Collection of Type Cultures (UK) as NCTC 13318(T).