Tumor necrosis factor receptor-associated factor 6 (TRAF6) participates in peroxinectin gene expression in Fenneropenaeus penicillatus
Tumor necrosis factor receptor-associated factor 6 (TRAF6) participates in peroxinectin gene expression in Fenneropenaeus penicillatus
复制标题
肿瘤坏死因子受体相关因子 6 (TRAF6) 参与长毛明对虾 peroxinectin 基因表达
DOI：
10.1016/j.fsi.2017.03.026
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发表时间：
2017-05-01
影响因子：
4.7
通讯作者：
Xu, Youhou
中科院分区：
文献类型：
--
作者：
Cai, Shuanghu;Huang, Yucong;Xu, Youhou
肿瘤坏死因子受体相关因子6(TRAF6)是一种重要的细胞质信号转接子，介导肿瘤坏死因子受体(TNFR)超家族和白介素1受体/Toll样受体(IL-1/TLR)超家族激活的信号。在本研究中，从细毛对虾中获得了TRAF6同源物(FpTRAF6)的全长cDNA。FpTRAF6的全长为2033bp，具有一个开放阅读框(ORF)，编码594个氨基酸，包括一个环型锌指，两个TRAF型锌指，以及一个保守的C-末端meprin和TRAF同源(MATH)结构域。FpTRAF6与其他TRAF6的氨基酸序列同源性在甲壳类为62.7%~94.1%，软体动物为45.6%~59.3%。实时荧光定量聚合酶链式反应表明，FpTRAF6在青霉各组织中均有结构性表达。微生物攻击后，FpTRAF6在不同组织中的表达模式不同。WSSV攻击后，FpTRAF6在心脏中表达下调，在鳃、肠和血细胞中无明显变化，在其他组织中表达上调。注射溶藻弧菌后，FpTRAF6在心脏和肠道中表达下调，在鳃、淋巴器官和造血器官中表达上调，在其他受试组织中无明显变化。用RNAi方法检测FpTRAF6的功能。结果表明，沉默FpTRAF6基因可在体内抑制Peroxinectin的表达，并增强对虾对WSSV和溶藻弧菌攻击的敏感性，提示FpTRAF6对细菌和病毒病原体具有积极的作用。综上所述，本研究结果对FpTRAF6在激活TLRs信号通路中的作用以及宿主对入侵病原体的防御提供了一些见解。(C)2017爱思唯尔有限公司。保留所有权利。
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is an important cytoplasm signal adaptor that mediates signals activated by tumor necrosis factor receptor (TNFR) superfamily and the Interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. In the study, the full-length cDNA of a TRAF6 homolog (FpTRAF6) was identified from Fenneropenaeus penicillatus. The full-length cDNA of FpTRAF6 is 2033 bp long, with an open reading frame (ORF) encoding a putative protein of 594 amino acids, including a RING type Zinc finger, two TRAF-type Zinc fingers, and a conserved C-terminal meprin and TRAF homology (MATH) domain. The overall amino acid sequence identity between FpTRAF6 and other TRAF6s ranged from 62.7 to 94.1% for crustaceans and from 45.6 to 59.3% for mollusca. Real-time qRT-PCR indicated that FpTRAF6 was constitutively expressed in various tissues of F. penicillatus. The temporal expression patterns of FpTRAF6 mRNA were different in the different tissues after microbial challenge. FpTRAF6 was downregulated in the heart, no obvious changes in the gill, intestine and hemocytes, and upregulated in other tested tissues after WSSV challenge. After V. alginolyticus injection, FpTRAF6 was downregulated in the heart and intestine, upregulated in the gill, lymphoid organ and hematopoietic organ, and no obvious changes in other tested tissues. RNAi assay was carried out to investigate the function of FpTRAF6. The results showed that silencing FpTRAF6 gene could inhibit peroxinectin expression in vivo, and enhance the sensitivity of shrimps to WSSV and V. alginolyticus challenge, suggesting FpTRAF6 could play a positive role against bacterial and viral pathogens. In conclusion, the results of the study provide some insights into the function of FpTRAF6 in activating TLRs signaling pathway and the host defense against invading pathogens. (C) 2017 Elsevier Ltd. All rights reserved.