Conditioned Place Avoidance of Zebrafish (Danio rerio) to Three Chemicals Used for Euthanasia and Anaesthesia
Conditioned Place Avoidance of Zebrafish (Danio rerio) to Three Chemicals Used for Euthanasia and Anaesthesia
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DOI：
10.1371/journal.pone.0088030
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发表时间：
2014-02-04
期刊：
影响因子：
3.7
通讯作者：
Weary, Daniel M.
中科院分区：
文献类型：
--
作者：
Wong, Devina;von Keyserlingk, Marina A. G.;Weary, Daniel M.
斑马鱼正在成为发育和生物医学研究中最常用的脊椎动物之一。在使用过量的甲磺酸三卡因（TMS，也称为 MS-222）的实验结束时，鱼通常会被杀死，但迄今为止，很少有研究评估接触这种药物或其他药物是否符合安乐死的条件（即“善意的死亡”）。替代药物包括盐酸美托咪酯和丁香油。我们使用条件性场所回避范式来比较对 TMS、丁香油和盐酸美托咪酯的厌恶。斑马鱼 (n = 51) 在明/暗箱最初首选的一侧暴露于不同的麻醉剂。暴露于 TMS 后，斑马鱼在其先前喜欢的一侧停留的时间减少了；接触盐酸美托咪酯和丁香油后，厌恶感不太明显。暴露于 TMS 的 17 条鱼中有 9 条选择不重新进入先前首选的一侧，而 18 条中的 2 条和 16 条中的 3 条分别拒绝盐酸美托咪酯和丁香油。我们得出的结论是，盐酸美托咪酯和丁香油比 TMS 更不令人厌恶，并且这些药物可用作 TMS 杀死斑马鱼的人道替代品。
Zebrafish are becoming one of the most used vertebrates in developmental and biomedical research. Fish are commonly killed at the end of an experiment with an overdose of tricaine methanesulfonate (TMS, also known as MS-222), but to date little research has assessed if exposure to this or other agents qualifies as euthanasia (i.e. a "good death"). Alternative agents include metomidate hydrochloride and clove oil. We use a conditioned place avoidance paradigm to compare aversion to TMS, clove oil, and metomidate hydrochloride. Zebrafish (n = 51) were exposed to the different anaesthetics in the initially preferred side of a light/dark box. After exposure to TMS zebrafish spent less time in their previously preferred side; aversion was less pronounced following exposure to metomidate hydrochloride and clove oil. Nine of 17 fish exposed to TMS chose not to re-enter the previously preferred side, versus 2 of 18 and 3 of 16 refusals for metomidate hydrochloride and clove oil, respectively. We conclude that metomidate hydrochloride and clove oil are less aversive than TMS and that these agents be used as humane alternatives to TMS for killing zebrafish.