Association between GSTP1 CpG methylation and the early phase of lead exposure
Association between GSTP1 CpG methylation and the early phase of lead exposure
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DOI：
10.3109/15376516.2013.859195
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发表时间：
2014-02
影响因子：
3.2
通讯作者：
Chunping Li;Xiaolin Yang;Ming Xu;Jinlong Zhang;N. Sun
中科院分区：
文献类型：
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作者：
Chunping Li;Xiaolin Yang;Ming Xu;Jinlong Zhang;N. Sun
摘要背景：谷胱甘肽S转移酶1是由铅诱导的，因此可以作为铅暴露的生物标志物。铅暴露改变DNA甲基化状态。目的：探讨谷胱甘肽S转移酶1（GST P1）基因启动子甲基化在铅中毒中的作用。材料与方法：我们对53名电池厂工人和53名年龄和性别匹配的健康志愿者进行了病例对照研究，以确定GST 1启动子甲基化水平是否与铅中毒风险相关。我们在细胞模型中采用甲基化特异性PCR（MSP）来确定GST P1甲基化水平与铅暴露之间的关系。结果：我们没有发现GST P1甲基化与铅暴露之间的关联。暴露组和对照组之间甲基化频率的差异无统计学意义（p = 0.401），具有甲基化GSTP 1基因的个体与铅中毒风险无关（校正OR = 1.36，95%CI，0.22-8.24）。结论：GST P1甲基化不参与铅中毒的早期阶段。GSTP 1甲基化与铅中毒风险的关系有待进一步研究。
Abstract Context: GSTP1 is induced by lead, and thus serves as a biomarker of lead exposure. Lead exposure changes DNA methylation status. Objective: We attempted to prove that the methylation of the GSTP1 promoter plays an important role in lead toxicity. Materials and methods: We conducted a case-control study of 53 workers from a battery plant and 53 age and sex matched healthy volunteers to determine whether the methylation level of the GSTP1 promoter is associated with the risk of lead poisoning. We employed methylation-specific PCR (MSP) in cell models to determine the relationship between the GSTP1 methylation level and lead exposure. Results: We found no association between GSTP1 methylation and lead exposure. The difference in methylation frequencies between the exposure group and the controls was not statistically significant (p = 0.401), and individuals with the methylated GSTP1 gene was not associated with the risk of lead poisoning (adjusted OR = 1.36, 95% CI, 0.22–8.24). Conclusion: This study suggests that GSTP1 methylation is not involved in the early phase of lead toxicity. Further studies should be performed to detect the association between GSTP1 methylation and the risk of lead poisoning in later phases.