A gradient-generating microfluidic device for cell biology.
A gradient-generating microfluidic device for cell biology.
复制标题
DOI：
10.3791/271
复制
发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Chung, Bong Geun;Manbachi, Amir;Khademhosseini, Ali
描述了用于研究细胞行为的梯度生成微流体装置的制造和操作。微流控平台是一种可行的实验工具，因为它可以精确地操纵流体流动，实现高通量实验，并产生稳定的可溶性浓度梯度。与传统的梯度发生器相比，基于聚（二甲基硅氧烷）（PDMS）的微流体装置可以产生具有明确轮廓的稳定的生长因子浓度梯度。在这里，我们开发了具有三个独立入口的简单梯度生成微流体装置。三个微通道合并为一个微通道以产生浓度梯度。生长因子梯度的稳定性和形状由异硫氰酸荧光素（FITC）-葡聚糖（其分子量与表皮生长因子（EGF）相似）证实。使用这种微流体装置，我们证明暴露于 EGF 浓度梯度的成纤维细胞会向更高浓度迁移。通过细胞追踪分析定量评估细胞迁移的方向和迁移细胞的运动性。因此，这种梯度生成微流体装置可能有助于研究和分析迁移细胞的行为。
The fabrication and operation of a gradient-generating microfluidic device for studying cellular behavior is described. A microfluidic platform is an enabling experimental tool, because it can precisely manipulate fluid flows, enable high-throughput experiments, and generate stable soluble concentration gradients. Compared to conventional gradient generators, poly(dimethylsiloxane) (PDMS)-based microfluidic devices can generate stable concentration gradients of growth factors with well-defined profiles. Here, we developed simple gradient-generating microfluidic devices with three separate inlets. Three microchannels combined into one microchannel to generate concentration gradients. The stability and shape of growth factor gradients were confirmed by fluorescein isothyiocyanate (FITC)-dextran with a molecular weight similar to epidermal growth factor (EGF). Using this microfluidic device, we demonstrated that fibroblasts exposed to concentration gradients of EGF migrated toward higher concentrations. The directional orientation of cell migration and motility of migrating cells were quantitatively assessed by cell tracking analysis. Thus, this gradient-generating microfluidic device might be useful for studying and analyzing the behavior of migrating cells.