Plasmonic and colloidal stability behaviours of Au-acrylic core-shell nanoparticles with thin pH-responsive shells.
Plasmonic and colloidal stability behaviours of Au-acrylic core-shell nanoparticles with thin pH-responsive shells.
复制标题
DOI：
10.1039/c8nr07440b
复制
发表时间：
2018-10
期刊：
影响因子：
6.7
通讯作者：
Shangli Wu;Mingning Zhu;Qing Lian;Dongdong Lu;B. Spencer;D. Adlam;J. Hoyland;Kirsten Volk;M. Karg;B. Saunders
中科院分区：
文献类型：
--
作者：
Shangli Wu;Mingning Zhu;Qing Lian;Dongdong Lu;B. Spencer;D. Adlam;J. Hoyland;Kirsten Volk;M. Karg;B. Saunders
Au纳米粒子的局域表面等离子体共振（LSPR）及其与邻近实体的相互作用提供了纳米尺度上丰富的基础和实用信息。许多研究已经研究了具有温度响应性的Au核和聚合物壳的核-壳NP。然而，有很少的研究pH值响应的Au-聚合物NP壳。沉淀聚合是一种可扩展的方法，在这里，我们建立了这样一种方法来合成pH响应性Au-聚（甲基丙烯酸甲酯）共聚物核-壳纳米粒子，而不需要预官能化。所用的共聚单体是甲基丙烯酸（MAA）或2-羧乙基丙烯酸酯（CEA）和壳与乙二醇二甲基丙烯酸酯交联。一系列的五个核-壳系统的坍塌壳厚度小于30 nm的研究。与使用沉淀聚合制备的相关Au-聚合物核-壳NP相比，基于CEA的核-壳NP的壳厚度相对较薄（≤5 nm）。核-壳纳米粒子的LSPR特性取决于壳的厚度，并成功地模拟使用时域有限差分（FDTD）计算。两个系统被认为是进一步作为范例。具有最厚壳的MAA基核壳体系对添加的电解质表现出增强的胶体稳定性。CEA为基础的核壳分散体与最薄的壳显示可逆的pH触发的聚集和细胞相容性的HeLa细胞。提供了证明细胞内pH报告的概念验证数据。
The localised surface plasmon resonance (LSPR) of Au nanoparticles (NPs) as well as its interaction with nearby entities provides a wealth of fundamental and practical information at the nanometre scale. A number of studies have investigated core-shell NPs with Au cores and polymer shells that are temperature-responsive. However, there are very few studies of pH-responsive Au-polymer NP shells. Precipitation polymerisation is a scalable method and here we establish such a method to synthesise pH-responsive Au-poly(methyl methacrylate) copolymer core-shell NPs without the need for pre-functionalisation. The comonomers used were methacrylic acid (MAA) or 2-carboxyethyl acrylate (CEA) and the shells were crosslinked with ethylene glycol dimethacrylate. A series of five core-shell systems with collapsed shell thicknesses less than 30 nm are studied. The shell-thicknesses for the CEA-based core-shell NPs are relatively thin (≤5 nm) compared to related Au-polymer core-shell NPs prepared using precipitation polymerisation. The LSPR properties of the core-shell NPs were dependent on the shell thickness and were successfully simulated using finite difference time domain (FDTD) calculations. Two systems are considered further as exemplars. The MAA-based core-shell system with the thickest shell exhibited enhanced colloidal stability to added electrolyte. The CEA-based core-shell dispersion with the thinnest shells displayed reversible pH-triggered aggregation and was cytocompatible for HeLa cells. Proof-of-concept data are presented that demonstrate intracellular pH reporting.