Seasonal variation in leaf glucosinolates and insect resistance in two types of Barbarea vulgaris ssp arcuata
Seasonal variation in leaf glucosinolates and insect resistance in two types of Barbarea vulgaris ssp arcuata
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DOI：
10.1016/s0031-9422(01)00151-0
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发表时间：
2001-09-01
期刊：
影响因子：
3.8
通讯作者：
Nielsen, JK
中科院分区：
文献类型：
--
作者：
Agerbirk, N;Olsen, CE;Nielsen, JK
从自然居群的叶子。检测了丹麦的黄蜀葵（蜀葵科）的芥子油苷含量和对十字花科特有的新金龟子Phyllotreta nemorum的抗性。该植物有两种类型（P型和G型）。G型叶含有芥子油苷（仅提及侧链）：（S）-2-羟基-2-苯乙基-（2S）、吲哚-3-基甲基-（4）和微量（R）-2-羟基-2-苯乙基-（2R）、2-苯乙基-（1）和4-甲氧基吲哚-3-基甲基（5）。P型叶以2R和4.并且仅含有痕量的1、2S和5，但还含有以前未知的（R）-2-羟基-2-（4-羟基苯基）乙基-（3R）。差向异构体（S）-2-羟基-2-（4-羟基苯基）乙基-（3S）在被认为是杂种的群体和B中发现。正岩2先令经核磁共振和质谱分析，分离鉴定了2R、2S、-2R、-3S和-3R，叶片中未检测到酰化硫代葡萄糖苷和烯丙基硫代葡萄糖苷。8月份硫甙含量变化较大。3-46多数种群硫甙含量较低，在3 - 15 μ mol/g干重之间。到11月35 - 75 μ mol/g干重%。G型在8、9月份对条跳甲新生幼虫具有抗性（3天存活率为0%），10、11月份逐渐丧失抗性（3天存活率为40-90%），硫甙含量与抗性无相关性。硫代葡萄糖苷也没有解释P型（总是敏感的）和G型（在夏季抵抗）之间的抗性差异。(C)2001爱思唯尔科技有限公司版权所有。
Leaves from natural populations of Barbarea vulgaris ssp. arcuata (Brassicaceae) in Denmark were examined for glucosinolate content and resistance to the crucifer specialist nea beetle Phyllotreta nemorum. Two types of the plant (P- and G-type) could be recognized. Leaves of the G-type contained the glucosinolates (only side chains mentioned): (S)-2-hydroxy-2-phenylethyl- (2S), indol-3-ylmethyl- (4) and in trace amount (R)-2-hydroxy-2-phenylethyl- (2R), 2-phenylethyl- (1) and 4-methoxyindol-3-ylmethyl(5). Leaves of the P-type were dominated by 2R and 4. and had only trace amounts of 1, 2S, and 5 but contained in addition the previously unknown (R)-2-hydroxy-2-(4-hydroxyphenyl)ethyl- (3R). The epimer, (S)-2-hydroxy-2-(4-hydroxyphenyl)ethyl- (3S) was found in populations believed to be hybrids, and in B. orthoccras. 2S. 2R, desulfo 2S, -2R, -3S and -3R were isolated and identified by NMR and MS. Acylated glucosinolates or allylglucosinolate were not detected in leaves. The glucosinolate content in August was variable. 3-46 mu mol/g dry wt, but was low in most populations, 3-15 mu mol/g dry wt, In general, the glucosinolate content increased during the autumn., to 35-75 mu mol/g dry %wt in November. The G-type was resistant to neonate larvae of Phyllotreta nemorum in August and September (survival in 3-day bioassay typically 0%), and gradually lost the resistance in October and November (survival in 3-day bioassay 40-90%), and there was no correlation between glucosinolate content and resistance. Neither did glucosinolates explain the difference in resistance between the P-type (always susceptible) and the G-type (resistant in the summer season). (C) 2001 Elsevier Science Ltd. All rights reserved.