Point-of-care rare cell cancer diagnostics.
Point-of-care rare cell cancer diagnostics.
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DOI：
10.1007/978-1-4939-2172-0_9
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Issadore, David
中科院分区：
文献类型：
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作者：
Issadore, David
从肿瘤脱落到外周循环的稀疏细胞是一种越来越有前途的资源，用于无创监测癌症进展，早期诊断疾病，并作为提高我们对癌症转移的理解的工具。然而，血液中循环肿瘤细胞（CTC）的浓度极低（7.5 mL血液中约1-100 CTC）以及它们的异质生物标志物表达限制了传统实验室技术对它们的检测。为了克服这些挑战，我们开发了一种基于微流控芯片的微型霍尔探测器（muHD），它可以直接测量全血中的单个免疫磁标记细胞。即使存在大量的血细胞和未结合的反应物，muHD也能检测到单个细胞，而且不需要任何洗涤或纯化步骤。此外，这种具有成本效益的单细胞分析技术非常适合小型化为低成本的移动平台。在本章中，我们描述了用于设计、制造和将这些芯片应用于癌症诊断的方法。
The sparse cells that are shed from tumors into peripheral circulation are an increasingly promising resource for noninvasive monitoring of cancer progression, early diagnosis of disease, and serve as a tool for improving our understanding of cancer metastasis. However, the extremely sparse concentration of circulating tumor cells (CTCs) in blood (~1-100 CTC in 7.5 mL of blood) as well as their heterogeneous biomarker expression has limited their detection using conventional laboratory techniques. To overcome these challenges, we have developed a microfluidic chip-based micro-Hall detector (muHD), which can directly measure single, immunomagnetically tagged cells in whole blood. The muHD can detect individual cells even in the presence of vast numbers of blood cells and unbound reactants, and does not require any washing or purification steps. Furthermore, this cost-effective, single-cell analytical technique is well suited for miniaturization into a mobile platform for low-cost point-of-care use. In this chapter, we describe the methodology used to design, fabricate, and apply these chips to cancer diagnostics.