Peripheral multidendritic sensory neurons are necessary for rhythmic locomotion behavior in Drosophila larvae
Peripheral multidendritic sensory neurons are necessary for rhythmic locomotion behavior in Drosophila larvae
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DOI：
10.1073/pnas.0700895104
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发表时间：
2007-03-20
影响因子：
11.1
通讯作者：
Jan, Yuh Nung
中科院分区：
文献类型：
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作者：
Song, Wei;Onishi, Maika;Jan, Yuh Nung
从呼吸到行走，有节奏的运动涵盖了整个动物王国的重要生理过程。许多人认为节奏行为的产生是由中枢模式发生器（CIPG）操作的，而不需要外围感觉输入。然而，需要感觉反馈来响应于实际运动的情况来修改或协调运动活动。与此相反，我们在这里报告说，感觉输入是必要的果蝇幼虫运动，一种形式的节奏行为的产生。阻断所有外周感觉输入导致幼虫爬行停止。通过有条件地沉默幼虫外周感觉神经元的各个子集，我们确定了多个树突状（MD）神经元的神经元产生的节奏蠕动。通过记录运动性运动活动，我们进一步证明，MD神经元输入的去除破坏了节律性运动放电模式，延长了刻板的节段性运动放电持续时间，并阻止了从后节段性运动放电到前节段性运动放电的传播。这些发现表明，MD感觉神经元输入是产生幼虫运动的神经回路中的必要组成部分。
From breathing to walking, rhythmic movements encompass physiological processes important across the entire animal kingdom. it is thought by many that the generation of rhythmic behavior is operated by a central pattern generator (CIPG) and does not require peripheral sensory input. Sensory feedback is, however, required to modify or coordinate the motor activity in response to the circumstances of actual movement. In contrast to this notion, we report here that sensory input is necessary for the generation of Drosophila larval locomotion, a form of rhythmic behavior. Blockage of all peripheral sensory inputs resulted in cessation of larval crawling. By conditionally silencing various subsets of larval peripheral sensory neurons, we identified the multiple dendritic (MD) neurons as the neurons essential for the generation of rhythmic peristaltic locomotion. By recording the locomotive motor activities, we further demonstrate that removal of MD neuron input disrupted rhythmic motor firing pattern in a way that prolonged the stereotyped segmental motor firing duration and prevented the propagation of posterior to anterior segmental motor firing. These findings reveal that MD sensory neuron input is a necessary component in the neural circuitry that generates larval locomotion.