Non-specific cytotoxic cell receptor (NCCRP)-1 type gene in tilapia (Oreochromis niloticus):: its cloning and analysis
Non-specific cytotoxic cell receptor (NCCRP)-1 type gene in tilapia (Oreochromis niloticus):: its cloning and analysis
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DOI：
10.1016/s1050-4648(03)00059-7
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发表时间：
2004-02-01
影响因子：
4.7
通讯作者：
Sakai, M
中科院分区：
文献类型：
--
作者：
Ishimoto, Y;Savan, R;Sakai, M
非特异性细胞毒性细胞相当于硬骨鱼的自然杀伤细胞。已知这些细胞介导鱼类的先天免疫反应。本研究从罗非鱼中克隆了一个由960 bps、12 bps 5' UTR、702 bps开放阅读框和246 bps 3' UTR组成的非特异性细胞毒性细胞受体蛋白（NCCRP） cDNA。预测基因产物为233个氨基酸，分子量为26.6 kDa， pI为6.07。该基因具有典型的F-box相关结构域，其中包含抗原结合区域，涉及与杀伤所需靶细胞结合的形成。半定量RT-PCR研究发现，NCCRP-1在肝脏中表达量最高，其次是头肾、脾和肠。在脑、鳃和心脏均有低表达。转录本在皮肤中表达最低。原位杂交证实，nccrp - 1转录本在淋巴细胞样细胞和中性粒细胞上均有表达。(C) 2003 Elsevier Ltd.版权所有。
Non specific cytotoxic cells are natural killer cell equivalent in teleosts. These cells are known to mediate innate immune responses in fishes. In this study, from tilapia, a non-specific cytotoxic cell receptor protein (NCCRP) like cDNA composed of 960 bps with a 12 bps 5' UTR, 702 bps open reading frame and 246 bps 3' UTR has been cloned. The predicted gene product is 233 amino acid with a molecular weight of 26.6 kDa and pI of 6.07. The gene has a typical F-box associated domain, which harbors the antigen binding region, implicated in formation of conjugates with target cells necessary for killing. By semi-quantitative RT-PCR studies, the expression level of NCCRP-1 was highest in liver followed by head kidney, spleen and intestine. Low expression levels were recorded in brain, gill and heart. Lowest expression of transcripts was in the skin. As evidenced by in situ hybridisation the NCCRP-I transcripts were expressed on lymphocyte like cells as well as neutrophils. (C) 2003 Elsevier Ltd. All rights reserved.