Analytical aspects in doping control: Challenges and perspectives
Analytical aspects in doping control: Challenges and perspectives
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DOI：
10.1016/j.forsciint.2011.07.024
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发表时间：
2011-12-10
影响因子：
2.2
通讯作者：
Veuthey, Jean-Luc
中科院分区：
文献类型：
--
作者：
Badoud, Flavia;Guillarme, Davy;Veuthey, Jean-Luc
自20世纪60年代首次进行反兴奋剂检测以来，检测的分析方面仍然具有挑战性。本文讨论了兴奋剂检测分析过程的演变，特别强调了分离技术，如气相色谱法和液相色谱法。这些方法正在改进，同时要求提高检测运动员生物样本中违禁物质的灵敏度和选择性。此外，必须进行快速分析，以处理不断增加的兴奋剂控制样本数量以及特定体育赛事期间所需的较短响应时间。质谱分析法的最新发展和精确质量测定的扩大改进了反兴奋剂战略，使之有可能利用元素组成和同位素模式进行结构鉴定，这些技术必须能够明确区分阴性和可疑样品，而不会出现假阴性或假阳性结果。因此，必须达到高度的可靠性，以确定对应于可疑分析物的主要代谢物。沿着制药工业的当前趋势，蛋白质和肽的分析仍然是兴奋剂控制中的一个重要问题。复杂的分析工具仍然是强制性的，以提高其与内源性类似物的区别。最后，将在反兴奋剂的背景下讨论间接方法，其中最新的进展旨在以全面的方式研究兴奋剂的生物反应。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Since the first anti-doping tests in the 1960s, the analytical aspects of the testing remain challenging. The evolution of the analytical process in doping control is discussed in this paper with a particular emphasis on separation techniques, such as gas chromatography and liquid chromatography. These approaches are improving in parallel with the requirements of increasing sensitivity and selectivity for detecting prohibited substances in biological samples from athletes. Moreover, fast analyses are mandatory to deal with the growing number of doping control samples and the short response time required during particular sport events. Recent developments in mass spectrometry and the expansion of accurate mass determination has improved anti-doping strategies with the possibility of using elemental composition and isotope patterns for structural identification.These techniques must be able to distinguish equivocally between negative and suspicious samples with no false-negative or false-positive results. Therefore, high degree of reliability must be reached for the identification of major metabolites corresponding to suspected analytes.Along with current trends in pharmaceutical industry the analysis of proteins and peptides remains an important issue in doping control. Sophisticated analytical tools are still mandatory to improve their distinction from endogenous analogs. Finally, indirect approaches will be discussed in the context of anti-doping, in which recent advances are aimed to examine the biological response of a doping agent in a holistic way. (C) 2011 Elsevier Ireland Ltd. All rights reserved.