Pseudomonas zhaodongensis sp. nov., isolated from saline and alkaline soils.
Pseudomonas zhaodongensis sp. nov., isolated from saline and alkaline soils.
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DOI：
10.1099/ijs.0.000057
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发表时间：
2015-03
影响因子：
2.8
通讯作者：
Lei Zhang;Yuanyuan Pan;Kaibiao Wang;Xiaoxia Zhang;Cheng Zhang;Shuang Zhang;Xiaowei Fu;Juquan Jiang
中科院分区：
文献类型：
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作者：
Lei Zhang;Yuanyuan Pan;Kaibiao Wang;Xiaoxia Zhang;Cheng Zhang;Shuang Zhang;Xiaowei Fu;Juquan Jiang
菌株NEAU-ST 5 -21（T）分离自黑龙江省肇东市盐碱土。革兰氏染色阴性，需氧，杆状，有极生鞭毛。它产生具有光滑表面的黄橙色菌落，并在0- 5%（w/v）NaCl（最佳0%，w/v）存在下，在20-40 °C（最佳28 °C）的温度和pH 7-11（最佳pH 7）下生长。基于分离的16 S rRNA基因序列和串联的16 S rRNA、gyrB和rpoD基因序列的系统发育分析表明，菌株NEAU-ST 5 -21（T）属于γ-变形菌纲假单胞菌属。最接近的物种是黄海假单胞菌，其模式菌株（KMM 1447（T））显示与菌株NEAU-ST 5 -21（T）的16 S rRNA、gyrB和rpoD的基因序列相似性为99.0%、81.8%和85.0%。菌株NEAU-ST 5 -21（T）与黄海假单胞菌DSM 18231（T）、昆明假单胞菌CGMCC 1.12273（T）、施氏假单胞菌DSM 5190（T）、噬油假单胞菌润滑假单胞菌DSM 21016（T）、成都假单胞菌CGMCC 2318（T）、嗜碱假单胞菌DSM 17744（T）和丰田假单胞菌DSM 26169（T）的耐药率为52± 0%至25± 2%。菌株NEAU-ST 5 -21（T）的DNA G+C含量为65mol%。主要脂肪酸（> 10%）为C18：1ω 7 c和/或C18：1ω6c、C16：1ω 7 c和/或C16：1ω6c和C16：0，主要呼吸醌为泛醌9，极性脂质由二磷脂酰甘油、磷脂酰乙醇胺、一种未知磷脂、磷脂酰甘油、一种未知氨基脂质、一种未知脂质和一种糖脂组成。拟命名为肇东假单胞菌Pseudomonaszhaodongensis sp. nov.，NEAU-ST 5 -21（T）（= ACCC 06362（T）= DSM 27559（T））是模式菌株。
Strain NEAU-ST5-21(T) was isolated from saline and alkaline soils in Zhaodong City, Heilongjiang Province, China. It was aerobic, Gram-stain-negative, rod-shaped and motile with a polar flagellum. It produced yellow-orange colonies with a smooth surface, and grew in the presence of 0-5 % (w/v) NaCl (optimum 0 %, w/v), at temperatures of 20-40 °C (optimum 28 °C) and at pH 7-11 (optimum pH 7). Phylogenetic analyses based on the separate 16S rRNA gene sequences and concatenated 16S rRNA, gyrB and rpoD gene sequences indicated that strain NEAU-ST5-21(T) belongs to the genus Pseudomonas in the class Gammaproteobacteria. The most closely related species is Pseudomonas xanthomarina, whose type strain (KMM 1447(T)) showed gene sequence similarities of 99.0 % for 16S rRNA, 81.8 % for gyrB and 85.0 % for rpoD with strain NEAU-ST5-21(T). DNA-DNA hybridization values between strain NEAU-ST5-21(T) and P. xanthomarina DSM 18231(T), Pseudomonas kunmingensis CGMCC 1.12273(T), Pseudomonas stutzeri DSM 5190(T), Pseudomonas oleovorans subsp. lubricantis DSM 21016(T), Pseudomomas chengduensis CGMCC 2318(T), Pseudomonas alcaliphila DSM 17744(T) and Pseudomonas toyotomiensis DSM 26169(T) were 52±0 % to 25±2 %. The DNA G+C content of strain NEAU-ST5-21(T) was 65 mol%. The major fatty acids (>10 %) were C18 : 1ω7c and/or C18 : 1ω6c, C16 : 1ω7c and/or C16 : 1ω6c and C16 : 0, the predominant respiratory quinone was ubiquinone 9, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid, phosphatidylglycerol, one unknown aminolipid, one unknown lipid and a glycolipid. The proposed name is Pseudomonas zhaodongensis sp. nov., NEAU-ST5-21(T) ( = ACCC 06362(T) = DSM 27559(T)) being the type strain.