Cryopreservation of periodontal ligament cells with magnetic field for tooth banking
Cryopreservation of periodontal ligament cells with magnetic field for tooth banking
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DOI：
10.1016/j.cryobiol.2010.05.003
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发表时间：
2010-08-01
期刊：
影响因子：
2.7
通讯作者：
Tanne, K.
中科院分区：
文献类型：
--
作者：
Kaku, M.;Kamada, H.;Tanne, K.
本研究的目的是建立一种可用于自体牙移植的牙齿长期冷冻保存方法。人牙周膜（PDL）细胞冷冻在10%的二甲基亚砜（Me2SO）使用程序冷冻机与磁场。将细胞在-150 ℃下冷冻保存7天。解冻后立即计数存活细胞数并培养细胞; 48 h后检查培养细胞。结果表明，0.01 mT的磁场，15分钟的保持时间，和30摄氏度的骤降温度导致PDL细胞的最大存活率。基于这些发现，将整个牙齿在相同条件下冷冻保存1年。器官培养结果显示，在无磁场的情况下，PDL细胞没有出现，但在有磁场的情况下，PDL细胞可以像新鲜牙齿一样增殖。组织学检查和透射电镜图像显示，冷冻保存的牙齿与磁场没有任何破坏冻存细胞。相比之下，在没有磁场的情况下冷冻的细胞中观察到严重的细胞损伤。这些结果表明，磁场程序冷冻机是可行的牙齿冷冻保存。(C)2010年爱思唯尔公司All rights reserved.
The purpose of this study was to establish a long-term tooth cryopreservation method that can be used for tooth autotransplantation. Human periodontal ligament (PDL) cells were frozen in 10% dimethyl sulfoxide (Me2SO) using a programmed freezer with a magnetic field. Cells were cryopreserved for 7 days at -150 degrees C. Immediately after thawing, the number of surviving cells was counted and the cells were cultured; cultured cells were examined after 48 h. Results indicated that a 0.01 mT of a magnetic field, a 15-min hold-time, and a plunging temperature of 30 degrees C led to the greatest survival rate of PDL cells. Based on these findings, whole teeth were cryopreserved under the same conditions for 1 year. The organ culture revealed that the PDL cells of cryopreserved tooth with a magnetic field could proliferate as much as a fresh tooth, although the cells did not appear in the cryopreserved tooth without a magnetic field. Histological examination and the transmission electron microscopic image of cryopreserved tooth with a magnetic field did not show any destruction of cryopreserved cells. In contrast, severe cell damage was seen in cells frozen without a magnetic field. These results indicated that a magnetic field programmed freezer is available for tooth cryopreservation. (C) 2010 Elsevier Inc. All rights reserved.