Evaluation of soil gram-negative bacteria yielding polyhydroxyalkanoic acids from carbohydrates and propionic acid
Evaluation of soil gram-negative bacteria yielding polyhydroxyalkanoic acids from carbohydrates and propionic acid
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DOI：
10.1007/s002530050763
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发表时间：
1996-07-01
影响因子：
5
通讯作者：
daSilva, LF
中科院分区：
文献类型：
--
作者：
Gomez, JGC;Rodrigues, MFA;daSilva, LF
筛选程序的开发导致75株土壤革兰氏阴性细菌的分离，能够产生聚羟基链烷酸（PHA）的甘蔗衍生物。对这些菌株进行了关于其将碳水化合物或丙酸转化为PHA组分的效率的评价。几种菌株能够利用蔗糖以及葡萄糖和果糖生长，然后以有希望的产量积累聚-（3-羟基丁酸）（PHB）。有7株菌株在转化碳水化合物为PHB时，其转化率超过理论值的80%，并积累了至少50%的细胞干重作为PHB。10个菌株将3-羟基戊酸单元从丙酸的聚合物中，其中3个产率与真养产碱杆菌的产率相当。
A screening programme was developed leading to the isolation of 75 strains of soil gram-negative bacteria which are able to produce polyhydroxyalkanoic acids (PHA) from sugar-cane derivatives. The evaluation of these strains was performed with regard to their efficiency in converting carbohydrates or propionic acid into PHA constituents. Several strains were able to use sucrose as well as glucose and fructose to grow and afterwards to accumulate poly-(3-hydroxybutyric acid) (PHB) with promising yields. Seven strains were found to have more than 80% of the theoretical value when converting carbohydrates into PHB and accumulated at least 50% of the cell dry weight as PHB. Ten strains incorporated 3-hydroxyvaleric acid units into the polymer from propionic acid of which 3 gave yields comparable to those of Alcaligenes eutrophus.