Determination and analysis of the dissipation and residue of cyprodinil and fludioxonil in grape and soil using a modified QuEChERS method
Determination and analysis of the dissipation and residue of cyprodinil and fludioxonil in grape and soil using a modified QuEChERS method
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DOI：
10.1007/s10661-015-4661-9
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发表时间：
2015-06
影响因子：
3
通讯作者：
Wen Zhang;Hongyu Chen;Xinwei Han;Zai-Bo Yang;Mingming Tang;Jing Zhang;Song Zeng;D. Hu;Kankan Zhang
中科院分区：
文献类型：
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作者：
Wen Zhang;Hongyu Chen;Xinwei Han;Zai-Bo Yang;Mingming Tang;Jing Zhang;Song Zeng;D. Hu;Kankan Zhang
建立了一种简便、准确的气相色谱-氮磷检测器联合检测葡萄和土壤中嘧菌腈和嘧菌腈的方法。通过日间和日间回收率实验，评价了该方法检测两种杀菌剂的准确度和精密度。检出限分别为0.017 mg/kg和0.030 mg/kg。葡萄和土壤中环虫腈的定量限分别为0.05 mg/kg和0.10 mg/kg。对葡萄和土壤中3个加标水平杀菌剂的加标回收率进行了研究，结果表明，环虫腈的加标回收率为85.81 ~ 102.94%，嘧菌腈的加标回收率为92.00 ~ 106.86%，相对标准偏差小于7%。在中国的两个试验点进行了田间试验。吡虫腈在葡萄中的半衰期为9.6 ~ 20.8 d，在土壤中的半衰期为5.8 ~ 15.6 d；氟菌腈在葡萄中的半衰期为6.2 ~ 7.2 d，在土壤中的半衰期为6.0 ~ 12.1 d。将62%水分散颗粒制剂低剂量喷3次，葡萄收获后14 d内，环虫腈和嘧菌腈的末端残留量均低于1.0 mg/kg。本研究可为建立环虫腈和嘧菌腈在葡萄中的最大残留限量，促进这两种杀菌剂的合理、安全使用提供参考。
A simple and accurate method coupled with a gas chromatography-nitrogen phosphorus detector was developed to detect cyprodinil and fludioxonil in grape and soil. The accuracy and precision of the method in detecting the two fungicides were evaluated by conducting intra- and inter-day recovery experiments. The limits of detection were 0.017 mg/kg for cyprodinil and 0.030 mg/kg for fludioxonil. The limits of quantitation were 0.05 mg/kg for cyprodinil and 0.10 mg/kg for fludioxonil in grape and soil. The recoveries of the fungicides in grape and soil were investigated at three spiked levels and were found to range from 85.81 to 102.94 % for cyprodinil and from 92.00 to 106.86 % for fludioxonil, with relative standard deviations below 7 %. Field experiments were conducted in two experimental locations in China. The half-lives of cyprodinil were 9.6–20.8 days in grape and 5.8–15.6 day in soil, and the half-lives of fludioxonil were 6.2–7.2 days in grape and 6.0–12.1 days in soil. When the cyprodinil and fludioxonil 62 % water-dispersible granule formulation was sprayed at a low dosage three times, terminal residues of cyprodinil and fludioxonil were below 1.0 mg/kg in grape 14 days after harvest. This work may serve as a reference to establish the maximum residue limits for cyprodinil and fludioxonil in grape and promote the proper and safe use of these two fungicides.