Isolation and characterization of a novel native Bacillus thuringiensis strain BRC‐HZM2 capable of degrading chlorpyrifos
Isolation and characterization of a novel native Bacillus thuringiensis strain BRC‐HZM2 capable of degrading chlorpyrifos
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DOI：
10.1002/jobm.201300501
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发表时间：
2015-03
影响因子：
3.1
通讯作者：
Songqing Wu;Yan Peng;Zhangmin Huang;Zhipeng Huang;Lei Xu;Gelbič Ivan;X. Guan;Lingling Zhang;Shuangquan Zou
中科院分区：
文献类型：
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作者：
Songqing Wu;Yan Peng;Zhangmin Huang;Zhipeng Huang;Lei Xu;Gelbič Ivan;X. Guan;Lingling Zhang;Shuangquan Zou
从毒死蜱污染的样品中分离出能降解苏云金芽孢杆菌(Bt)的毒死蜱菌株。用改良醋酸钠抗生素热处理法分离到6株Bt菌株(分离率为2.7%)，筛选出1株新菌株(BRC-HZM2)进行分析。从生化反应、抗生素敏感性、16S rRNA基因、质粒谱、杀虫晶体蛋白谱、Cys和Cyt基因的PCR-RFLP等方面对该菌株进行了表型和系统发育分析。测定了BRC-HZM2菌株在液体培养48h内对毒死蜱的降解速率。结果表明，Bt菌株在12h内降解了50%以上的毒死蜱，48h后降解率为88.9%，这些结果突出了Bt菌株在生物防治和环境修复方面的潜力。
Studies were carried out to isolate chlorpyrifos degrading Bacillus thuringiensis (Bt) strains from chlorpyrifos‐contaminated samples. Six Bt strains (isolation rate 2.7%) were isolated by modified sodium acetate antibiotic heat treatment, and one novel strain (BRC‐HZM2) was selected for further analysis. Phenotype and phylogeny analysis of this strain was conducted on the basis of biochemical reactions, antibiotic sensitivity, 16s rRNA genes, plasmid profile, insecticidal crystal protein profiles, and PCR–RFLP for cry and cyt genes. The degradation rate of chlorpyrifos in liquid culture was estimated during 48 h of incubation for the isolate BRC‐HZM2. More than 50% of the initial chlorpyrifos concentration degraded within 12 h, 88.9% after 48 h. These results highlight the potential of the Bt strain for biological control and the bioremediation of environments contaminated with chlorpyrifos.