Effects of asbestos fibers on cell division, cell survival, and formation of thioguanine-resistant mutants in Chinese hamster ovary cells.
Effects of asbestos fibers on cell division, cell survival, and formation of thioguanine-resistant mutants in Chinese hamster ovary cells.
复制标题
石棉纤维对中国仓鼠卵巢细胞细胞分裂、细胞存活和硫鸟嘌呤抗性突变体形成的影响。
DOI：
10.1016/s0013-9351(86)80069-x
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发表时间：
1986
影响因子：
8.3
通讯作者：
N. Ringertz
中科院分区：
文献类型：
--
作者：
K. Kenne;S. Ljungquist;N. Ringertz
在细胞培养物中检查了青石棉纤维在中国仓鼠卵巢（CHO）细胞中诱导点突变和有丝分裂异常的能力。目的是研究建立体外测试方法来量化石棉和其他矿物纤维引起的遗传损伤的可能性。用CHO/次黄嘌呤鸟嘌呤磷酸核糖基转移酶系统获得的结果表明，青石棉纤维本身并没有显着增加硫鸟嘌呤抗性突变体的数量。青石棉纤维也未能增强苯并[a]芘的致突变性。延时摄影和显微镜显示石棉（青石棉）纤维具有明显的细胞毒性。在存活的细胞中，一些细胞经历了异常细胞分裂，产生了多核和微核细胞。然而，许多含有少量石棉纤维的细胞经历了有丝分裂，并成功形成了两个能够进一步分裂的单核子细胞。通过延时电视录像检查 4-5 天的单个含纤维细胞。在此期间，一些细胞经历了六次分裂并产生了几乎正常数量的子细胞。含有更长或等于有丝分裂细胞直径（20μm）的纤维的细胞表现出不同形式的有丝分裂异常。接触石棉纤维后，多核细胞的频率急剧增加。然而，这些细胞很少分裂产生能够持续细胞增殖的活子细胞。多核细胞的频率取决于接触石棉纤维的剂量，并且可能用作矿物纤维引起的有丝分裂紊乱程度的指标。
The ability of crocidolite fibers to induce point mutations and mitotic abnormalities in Chinese hamster ovary (CHO) cells was examined in cell cultures. The purpose has been to study the possibilities for establishingin vitrotest methods to quantify genetic damage induced by asbestos and other mineral fibers. Results obtained with the CHO/hypoxanthine guanine phosphoribosyl transferase system indicated that crocidolite fibersper sedo not significantly increase the number of thioguanine-resistant mutants. Crocidolite fibers also failed to potentiate the mutagenicity of benzo[a]pyrene. Time-lapse cinematography and microscopy showed that asbestos (crocidolite) fibers were markedly cytotoxic. Among surviving cells some underwent abnormal cell divisions which resulted in multi- and micronucleate cells. Many cells that contained a few asbestos fibers, however, underwent mitosis and successfully formed two mononucleate daughter cells capable of further divisions. Individual, fiber-containing cells were examined by time-lapse television recordings for 4–5 days. During this time period some cells underwent six divisions and generated an almost normal number of daughter cells. Cells which contained fibers that were longer or equivalent to the diameter of the mitotic cell (20 μm), showed different forms of mitotic abnormalities. The frequency of multinucleate cells was drastically increased following exposure to asbestos fibers. Only rarely, however, did these cells divide to produce viable daughter cells capable of continued cell multiplication. The frequency of multinucleate cells was dependent on the dose of exposure to asbestos fibers and could possibly be used as an index of the degree of mitotic disturbances induced by mineral fibers.