Periodontal disease at the biofilm-gingival interface
Periodontal disease at the biofilm-gingival interface
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DOI：
10.1902/jop.2007.060465
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发表时间：
2007-10-01
影响因子：
4.3
通讯作者：
Beck, J. D.
中科院分区：
文献类型：
--
作者：
Offenbacher, S.;Barros, S. P.;Beck, J. D.
背景：一项分子流行病学研究提供了描述 6,768 名社区居民受试者生物膜-牙龈界面 (BGI) 生物学特征的机会。方法：利用临床、微生物、炎症和宿主反应数据开发疾病分类和多变量模型。目的是根据 8 种牙菌斑细菌的 DNA 棋盘分析、17 种细菌的血清免疫球蛋白 G (IgG) 滴度以及 16 种炎症介质的龈沟液 (GCF) 水平，确定代表不同生物表型的新临床类别。使用探诊深度 (PD) 和探诊出血 (BOP) 评分定义了五种 BGI 临床病症。所有 PD = 4 mm [深部病变 (DL)] 的受试者分为低 BOP (18.0%)、中度 BOP (BGI-DL/MB, 39.7%) 和重度 BOP (BGI-DL/SB, 12.9%)。结果：BGI-G 受试者的直肠弯曲杆菌特异性血清 IgG 水平升高（P = 0.01），而 BGI-DL/SB 受试者的牙龈卟啉单胞菌（P < 0.0003）和直肠弯曲杆菌（P < 0.01）的 IgG 水平升高。 BGI-DL/SB 受试者的 GCF 白细胞介素 (IL)-1 β 和前列腺素 E-2 反应过度，慢性炎症反应增强，GCF IL-6 和单核细胞趋化肽-1 显着增加。在 BGI-wDL/SB 受试者中，更严重的口袋形成和 BOP 与更高水平的 GCF IL-1 β 相关，而不是与更高的微生物计数或菌斑评分相关。结论：新的 BGI 分类创建了具有不同生物学表型的类别。 68.5% 的 BGI-G 受试者中直肌 IgG 滴度升高，BGI-DL/MB 和 BGI-DL/SB 受试者中牙龈卟啉单胞菌滴度升高（分别为 63.8% 和 75.7%），强烈支持 BGI 类别发病机制的微生物特异性。
Background: A molecular epidemiologic study provided the opportunity to characterize the biology of the biofilm-gingival interface (BGI) in 6,768 community-dwelling subjects.Methods: Disease classifications and multivariable models were developed using clinical, microbial, inflammatory, and host-response data. The purpose was to identify new clinical categories that represented distinct biologic phenotypes based upon DNA checkerboard analyses of eight plaque bacteria, serum immunoglobulin G (IgG) titers to 17 bacteria, and the gingival crevicular fluid (GCF) levels of 16 inflammatory mediators. Five BGI clinical conditions were defined using probing depths (PDs) and bleeding on probing (BOP) scores. Subjects with all PDs = 4 mm [deep lesion (DL)] were divided into low BOP (18.0%), moderate BOP (BGI-DL/MB, 39.7%), and severe BOP (BGI-DL/SB, 12.9%). Results: Subjects with BGI-G had increased levels of Campylobacter rectus-specific serum IgG levels (P = 0.01), and those with BGI-DL/SB had increased IgG levels to Porphyromonas gingivalis (P < 0.0003) and C rectus (P < 0.01). BGI-DL/SB subjects had an excessive GCF interleukin (IL)-1 beta and prostaglandin E-2 response and an enhanced chronic inflammatory response with significant increases in GCF IL-6 and monocyte chemotactic peptide-1. Within BGI-wDL/SB subjects, more severe pocketing and BOP were associated with higher levels of GCF IL-1 beta, not higher microbial counts or plaque scores.Conclusions: New BGI classifications create categories with distinct biologic phenotypes. The increased titers of C rectus IgG among 68.5% of the BGI-G subjects and elevated P. gingivalis titers among BGI-DL/MB and BGI-DL/SB subjects (63.8% and 75.7%, respectively) are strongly supportive of the microbial specificity of pathogenesis for BGI categories.