The Aspergillus nidulans acuL gene encodes a mitochondrial carrier required for the utilization of carbon sources that are metabolized via the TCA cycle
The Aspergillus nidulans acuL gene encodes a mitochondrial carrier required for the utilization of carbon sources that are metabolized via the TCA cycle
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DOI：
10.1016/j.fgb.2014.04.012
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发表时间：
2014-07-01
影响因子：
3
通讯作者：
Velot, Christian
中科院分区：
文献类型：
--
作者：
Flipphi, Michel;Oestreicher, Nathalie;Velot, Christian
在中性曲霉（Aspergillus nidulans）中，醋酸作为唯一碳源的利用需要多个基因（acu）。它们中的大多数也是脂肪酸利用所必需的。这是acuD和acuE的情况，它们分别编码两种glyoxylate环特异性酶，异柠檬酸裂解酶和苹果酸合成酶，以及我们鉴定为AN7287的acuL，并在本研究中进行了表征。acuL的缺失导致与原始acuL217突变体相同的表型。acuL编码一个322个氨基酸的蛋白，具有线粒体膜载体的所有结构特征，与Saccharomyces cerevisiae琥珀酸/富马酸线粒体反转运蛋白Sfc1p（也称为Acr1p）具有60%的一致性。与此一致的是，AcuL蛋白被证明定位于线粒体，并且在酿酒葡萄球菌和空心葡萄球菌的同源物之间观察到部分交叉互补。广泛的表型特征表明，acuL基因参与碳源的利用，这些碳源通过TCA循环分解代谢，因此需要糖异生。此外，acuL被证明与acuD和acuE共同调节。总的来说，我们的数据表明AcuL可以通过将细胞质琥珀酸交换为线粒体富马酸，将乙醛酸循环与糖异生联系起来。(C) 2014爱思唯尔公司版权所有。
In Aspergillus nidulans, the utilization of acetate as sole carbon source requires several genes (acu). Most of them are also required for the utilization of fatty acids. This is the case for acuD and acuE, which encode the two glyoxylate cycle-specific enzymes, isocitrate lyase and malate synthase, respectively, but also for acuL that we have identified as AN7287, and characterized in this study. Deletion of acuL resulted in the same phenotype as the original acuL217 mutant. acuL encodes a 322-amino acid protein which displays all the structural features of a mitochondrial membrane carrier, and shares 60% identity with the Saccharomyces cerevisiae succinate/fumarate mitochondrial antiporter Sfc1p (also named Acr1p). Consistently, the AcuL protein was shown to localize in mitochondria, and partial cross-complementation was observed between the S. cerevisiae and A. nidulans homologues. Extensive phenotypic characterization suggested that the acuL gene is involved in the utilization of carbon sources that are catabolized via the TCA cycle, and therefore require gluconeogenesis. In addition, acuL proves to be co-regulated with acuD and acuE. Overall, our data suggest that AcuL could link the glyoxylate cycle to gluconeogenesis by exchanging cytoplasmic succinate for mitochondrial fumarate. (C) 2014 Elsevier Inc. All rights reserved.