Adsorption of lysozyme on base metal surfaces in the presence of an external electric potential
Adsorption of lysozyme on base metal surfaces in the presence of an external electric potential
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DOI：
10.1016/j.colsurfb.2016.07.042
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发表时间：
2016-11-01
影响因子：
5.8
通讯作者：
Imamura, Koreyoshi
中科院分区：
文献类型：
--
作者：
Htwe, Ei Ei;Nakama, Yuhi;Imamura, Koreyoshi
研究了外电势对蛋白质在贱金属表面吸附的影响。以鸡蛋白色溶菌酶（LSZ）为蛋白质载体，以不锈钢SUS316 L（St）、Ti、Ta、Zr、Cr、Ni等6种金属为基体，分别作为蛋白质载体和吸附载体。允许LSZ在不同条件（表面电位、pH、电解质类型和浓度、表面材料）下吸附在表面上，这使用椭圆偏振仪进行监测。LSZ吸附最小化在一定的阈值以上的电位范围内，并在低于阈值的表面电位范围内，降低表面电位增加的蛋白质吸附量。LSZ吸附的阈值电位随着pH值的增加而向正值移动，Ta和Zr的吸附阈值电位低于其他两种。二价阴离子盐（K2SO4）作为电解质在正电位范围内表现出LSZ的吸附，而一价盐（KCl）则没有。综合考虑所获得的结果表明，两种相互作用模式，即由外部电场产生的电力和与电离表面羟基的静电相互作用，作用于LSZ分子并确定LSZ吸附的抑制程度，所有这些发现似乎支持这样的观点，即可以通过操纵表面来控制贱金属表面对蛋白质的亲和力电势，如已经报道的一些电极材料上的电势。(C)2016爱思唯尔B.V.保留所有权利。
The impact of external electric potential on the adsorption of a protein to base metal surfaces was examined. Hen egg white lysozyme (LSZ) and six types of base metal plates (stainless steel SUS316L (St), Ti, Ta, Zr, Cr, or Ni) were used as the protein and adsorption surface, respectively. LSZ was allowed to adsorb on the surface under different conditions (surface potential, pH, electrolyte type and concentration, surface material), which was monitored using an ellipsometer. LSZ adsorption was minimized in the potential range above a certain threshold and, in the surface potential range below the threshold, decreasing the surface potential increased the amount of protein adsorbed. The threshold potential for LSZ adsorption was shifted toward a positive value with increasing pH and was lower for Ta and Zr than for the others. A divalent anion salt (K2SO4) as an electrolyte exhibited the adsorption of LSZ in the positive potential range while a monovalent salt (KCI) did not. A comprehensive consideration of the obtained results suggests that two modes of interactions, namely the electric force by an external electric field and electrostatic interactions with ionized surface hydroxyl groups, act on the LSZ molecules and determine the extent of suppression of LSZ adsorption, All these findings appear to support the view that a base metal surface can be controlled for the affinity to a protein by manipulating the surface electric potential as has been reported on some electrode materials. (C) 2016 Elsevier B.V. All rights reserved.