Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration
Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration
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DOI：
10.1016/j.febslet.2012.11.013
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发表时间：
2013-01-16
期刊：
影响因子：
3.5
通讯作者：
Hordijk, Peter L.
中科院分区：
文献类型：
--
作者：
Lam, B. Daniel;Anthony, Eloise C.;Hordijk, Peter L.
RhoGTTR Rac 1以极化方式激活并控制细胞运动性。我们以前表明，Rac1结合PP2A抑制剂SET和招聘核SET的胞质溶胶。我们发现，一个SET突变体，缺乏核定位信号，SET（三角洲NLS），促进细胞扩散和运动。这是伴随着膜皱褶的数量和频率的增加。PP2A的药理学抑制并不模拟SET（Delta NLS）的作用，然而，我们发现SET和SET（Delta NLS）的表达增加了MAP激酶ERK 1和ERK 2的水平。蛋白质相互作用的结构化总结：RAC 1通过下拉与SET物理相互作用（查看相互作用）。(C)2012年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(Delta NLS), promotes cell spreading and motility. This was accompanied by an increase in the number and frequency of membrane ruffles. Pharmacological inhibition of PP2A did not mimic the effects of SET(Delta NLS), however, we found that expression of SET and SET(Delta NLS) increases the levels of the MAP kinases ERK1 and ERK2.Structured summary of protein interactions:RAC1 physically interacts with SET by pull down (View interaction). (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.