Age-dependent homeostatic plasticity of GABAergic signaling in developing retinal networks.
Age-dependent homeostatic plasticity of GABAergic signaling in developing retinal networks.
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DOI：
10.1523/jneurosci.3112-11.2011
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发表时间：
2011-08-24
期刊：
影响因子：
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通讯作者：
Sernagor E
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文献类型：
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作者：
Hennig MH;Grady J;van Coppenhagen J;Sernagor E
发育中的视网膜神经节细胞以相关的自发爆发的方式发射，从而产生具有跨发育和物种保存的强大时空特征的传播波。在这里，我们调查的影响，稳态适应的电路控制视网膜波。小鼠视网膜波记录在体外长达35小时的GABAA拮抗剂荷包牡丹碱的存在下，用多电极阵列，使我们能够获得一个精确的，时间分辨的表征在此制备的稳态作用。实验在P4-P6（当GABAA信号传导在神经节细胞中去极化时）和P7-P10（当GABAA信号传导超极化时）进行。在所有年龄段，荷包牡丹碱最初增加了波的大小和其他活动指标。在P5-P6，波的大小在几个小时内下降到控制水平，而放电仍然很强，但这种补偿能力从P7开始完全消失。这表明自发视网膜活动的稳态控制以年龄依赖性方式维持特定的网络动态特性，并表明潜在机制与GABAA信号传导有关。
Developing retinal ganglion cells fire in correlated spontaneous bursts, resulting in propagating waves with robust spatiotemporal features preserved across development and species. Here we investigate the effects of homeostatic adaptation on the circuits controlling retinal waves. Mouse retinal waves were recorded in vitro for up to 35 h with a multielectrode array in presence of the GABAA antagonist bicuculline, allowing us to obtain a precise, time-resolved characterization of homeostatic effects in this preparation. Experiments were performed at P4–P6, when GABAA signaling is depolarizing in ganglion cells, and at P7–P10, when GABAA signaling is hyperpolarizing. At all ages, bicuculline initially increased the wave sizes and other activity metrics. At P5–P6, wave sizes decreased toward control levels within a few hours while firing remained strong, but this ability to compensate disappeared entirely from P7 onwards. This demonstrates that homeostatic control of spontaneous retinal activity maintains specific network dynamic properties in an age-dependent manner, and suggests that the underlying mechanism is linked to GABAA signaling.