Podoplanin expression profiles characteristic of odontogenic tumor-specific tissue architectures
Podoplanin expression profiles characteristic of odontogenic tumor-specific tissue architectures
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DOI：
10.1016/j.prp.2011.12.016
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Saku, Takashi
中科院分区：
文献类型：
--
作者：
Tsuneki, Masayuki;Maruyama, Satoshi;Saku, Takashi
平足蛋白（Podoplanin）是淋巴管内皮细胞具有代表性的免疫组化标志物，尽管其病理生理功能在很大程度上尚不明确，但它也在许多其他类型的癌细胞中表达。我们的目的是确定平足蛋白在牙源性肿瘤中的免疫定位模式，以探讨平足蛋白在其特征性组织结构形成中可能发挥的作用。我们对成釉细胞瘤（AM）、牙源性腺样瘤（AOT）、牙源性钙化囊性瘤（CCOT）和牙源性角化囊性瘤（KCOT）的40例手术标本中的平足蛋白免疫组化特征进行了研究，并与增殖细胞核抗原（PCNA）、整合素β1、纤连蛋白和基质金属蛋白酶9（MMP - 9）的免疫组化特征进行了比较。平足蛋白定位于成釉细胞瘤病灶的基底细胞层或周边区域。它存在于牙源性腺样瘤的梭形肿瘤细胞中，牙源性钙化囊性瘤的基底细胞和多面体细胞中，以及牙源性角化囊性瘤衬里的基底细胞和副基底细胞中。平足蛋白阳性（+）细胞位于PCNA +细胞区域内，整合素β1定位于平足蛋白+细胞的细胞膜上，位于纤连蛋白和MMP - 9沉积的细胞间隙中。总之，牙源性肿瘤中的平足蛋白+细胞和区域与细胞外基质信号传导以及细胞增殖密切相关。（C）2012爱思唯尔集团。保留所有权利。
Podoplanin, a representative immunohistochemical marker for lymphatic endothelial cells, is also expressed in many other kinds of cancer cells, although its pathophysiological function is largely unknown. Our aim was to determine immunolocalization modes of podoplanin among odontogenic tumors to discuss possible roles of podoplanin in their characteristic tissue architecture formation. Immunohistochemical profiles of podoplanin were investigated in 40 surgical specimens from ameloblastoma (AM), adenomatoid odontogenic tumor (AOT), calcifying cystic odontogenic tumor (CCOT), and keratocystic odontogenic tumor (KCOT) in comparison with those of proliferating cell nuclear antigen (PCNA), integrin beta 1, fibronectin, and matrix metalloproteinase 9 (MMP-9). Podoplanin was localized in the basal cell layer or in the peripheral zone of AM foci. It was found in spindle-shaped tumor cells of AOT, in both the basal and polyhedral cells of CCOT, and in the basal and parabasal cells of KCOT linings. Podoplanin-positive (+) cells were located within areas of PCNA+ cells, and integrin beta 1 was localized in the cell membrane of podoplanin+ cells in the intercellular space where fibronectin and MMP-9 were deposited. In conclusion, podoplanin+ cells and areas in odontogenic tumors are in close associations with extracellular matrix signalings as well as cell proliferation. (C) 2012 Elsevier GmbH. All rights reserved.