Rapid Oocyte Growth and Artificial Fertilization of the Larvaceans Oikopleura dioica and Oikopleura longicauda
Rapid Oocyte Growth and Artificial Fertilization of the Larvaceans Oikopleura dioica and Oikopleura longicauda
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幼虫 Oikopleura dioica 和 Oikopleura longicauda 卵母细胞的快速生长和人工受精
DOI：
10.2108/zsj.15.723
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
M. Morisawa
中科院分区：
文献类型：
--
作者：
A. Nishino;M. Morisawa
本文介绍了异卵厚壳蟹Oikopleura dioica和厚壳厚壳蟹Oikopleura dioica的快速配子生长和人工受精方法。长尾（Oikopleuridae科：附尾纲：尾索动物亚门）。在这些物种中，卵母细胞生长非常迅速，从直径约40 μm到约75 μm（O. dioica）和110 μm（O. longicauda），在生长末期切除生殖腺可获得成熟的配子。通过性腺解剖获得的卵子和精子混合在一起可以受精。
Abstract We describe here rapid gamete growth and artificial fertilization method of species of the larvaceans (appendicularians), Oikopleura dioica and O. longicauda (Family Oikopleuridae: Class Appendicularia: Subphylum Urochordata). In these species, oocytes grew very rapidly from about 40 μm in diameter to about 75 μm (O. dioica) and 110 μm (O. longicauda), respectively within a few hr. Moreover, cutting off the gonads at the last phase of the growth stage yielded matured gametes. The eggs and sperm obtained by the dissection of gonads could be fertilized when they were mixed together.