SOME CHANGES IN REACTIVITY OF ENZYMES RESULTING FROM THEIR CHEMICAL ATTACHMENT TO WATER-INSOLUBLE DERIVATIVES OF CELLULOSE
SOME CHANGES IN REACTIVITY OF ENZYMES RESULTING FROM THEIR CHEMICAL ATTACHMENT TO WATER-INSOLUBLE DERIVATIVES OF CELLULOSE
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DOI：
10.1042/bj1070669
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发表时间：
1968-01-01
影响因子：
4.1
通讯作者：
CROOK, EM
中科院分区：
文献类型：
--
作者：
HORNBY, WE;LILLY, MD;CROOK, EM
1. Purified ficin was chemically attached to CM-cellulose, and partially purified ATP–creatine phosphotransferase was chemically attached to both CM-cellulose andp-aminobenzylcellulose. 2. The apparentKmwith respect to ATP and Mg2+of ATP–creatine phosphotransferase was observed to increase about tenfold on attachment of the enzyme to CM-cellulose, and to increase by only 23% on its attachment top-aminobenzylcellulose. 3. The reactivity of both ficin and ATP–creatine phosphotransferase with 5,5′-dithiobis-(2-nitrobenzoic acid) was observed to decrease on chemical attachment of these enzymes to water-insoluble derivatives of cellulose. With derivatives prepared from CM-cellulose, the extent of the reaction with 5,5′-dithiobis-(2-nitrobenzoic acid) was dependent on ionic strength, but with similar derivatives prepared fromp-aminobenzylcellulose the extent of this reaction was independent of ionic strength. 4. The effect of diffusion and electrostatic interaction of charged enzyme substrates and charged enzyme supports on the apparentKmof a water-insoluble derivative of an enzyme is discussed. An equation is derived that satisfactorily describes the observed effects of these factors on the apparentKm.
