Improvement of embryogenic callus induction and shoot regeneration of buffalograss by silver nitrate
Improvement of embryogenic callus induction and shoot regeneration of buffalograss by silver nitrate
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DOI：
10.1023/a:1006468324616
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发表时间：
2000-01-01
影响因子：
3
通讯作者：
Riordan, TP
中科院分区：
文献类型：
--
作者：
Fei, SZ;Read, PE;Riordan, TP
在愈伤组织诱导培养基中添加乙烯拮抗剂银银（AgNO 3），显著提高了野牛草NE 84 -45-3和“Texoka”的田间雄性未成熟花序培养物的胚性愈伤组织产生（诱导频率和愈伤组织生长）。AgNO 3对‘609’和‘Texoka’的雌株幼穗胚性愈伤组织诱导没有明显的促进作用。愈伤组织在含AgNO 3的培养基上诱导的芽再生愈伤组织比在不含AgNO 3的培养基上诱导的芽再生愈伤组织多。无论愈伤组织来源如何，2.2 μ M的苄基腺嘌呤对再生的反应最好。虽然平均芽再生每个愈伤组织的数目较低的愈伤组织上开始含AgNO 3的培养基，芽再生的总数较高。然而，刺激效应是环境和基因型依赖性的。虽然添加AgNO 3显着刺激胚性愈伤组织诱导的NE 84 -45-3未成熟的花序收集在1995年秋季和1997年5月，它只略有增加胚性愈伤组织诱导频率在1996年5月时，多雨的条件下发生。对于雄花序的'Texoka'收集在5月初，硝酸银显着增强胚性愈伤组织的生产一贯在两年期间（1996年，1997年）。
Addition of the ethylene antagonist, silver nitrate (AgNO3), into callus induction medium significantly enhanced embryogenic callus production (both induction frequency and callus growth) of field-collected male immature inflorescence cultures of buffalograss NE84-45-3 and 'Texoka'. No stimulatory effect of AgNO3 was observed on embryogenic callus induction for female immature inflorescence culture of a female genotype '609' and 'Texoka'. Calli initiated on AgNO3-containing media had more shoot-regenerating calli than those initiated on AgNO3-free media, when they were transferred to the regeneration media. Benzyladenine at 2.2 mu M gave the best response for regeneration, regardless of the callus source. Although average number of shoots regenerated per callus was lower for calli initiated on AgNO3-containing media, total number of shoots regenerated was higher. The stimulatory effect, however, was environment and genotype dependent. While the addition of AgNO3 significantly stimulated embryogenic callus induction of NE84-45-3 immature inflorescences collected in Fall 1995 and May 1997, it only slightly increased the embryogenic callus induction frequencies in May 1996 when rainy conditions occurred. For male inflorescences of 'Texoka' collected in early May, AgNO3 significantly enhanced embryogenic callus production consistently over the two-year period (1996, 1997).