EXPRESSION OF THE NOVEL BASIC HELIX-LOOP-HELIX GENE EHAND IN NEURAL CREST DERIVATIVES AND EXTRAEMBRYONIC MEMBRANES DURING MOUSE DEVELOPMENT
EXPRESSION OF THE NOVEL BASIC HELIX-LOOP-HELIX GENE EHAND IN NEURAL CREST DERIVATIVES AND EXTRAEMBRYONIC MEMBRANES DURING MOUSE DEVELOPMENT
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DOI：
10.1006/dbio.1995.1245
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发表时间：
1995-08-01
影响因子：
2.7
通讯作者：
OLSON, EN
中科院分区：
文献类型：
--
作者：
CSERJESI, P;BROWN, D;OLSON, EN
我们采用酵母双杂交技术筛选小鼠胚胎cDNA文库的新的组织特异性B类碱性螺旋-环-螺旋（bHLH）转录因子，异源二聚体与广泛表达的A类bHLH蛋白E12。从这个筛选中，我们克隆了一个新的bHLH蛋白，我们命名为eHAND。它与其他bHLH家族成员的低序列同一性和发育过程中独特的表达模式表明，eHAND定义了一个新的B类bHLH蛋白亚类。在整个发育过程中，eHAND在滋养层细胞和胚外膜中以高水平表达。胚胎中eHAND表达的第一个位点是心脏，在心脏中，它在胚胎发育后8.5至10.5天（d.p.c.）之间以高水平表达，之后转录水平突然下降。到13.5 d.p.c.，eHAND在心脏中的表达定位于瓣膜形成区域，在胚胎的其他区域中的表达局限于具有实质性神经嵴组分的组织，eHAND在第一鳃弓及其衍生物、交感肾上腺谱系和肠系统中表达。在发育过程中的表达模式的eHAND是不同于其他的bHLH基因，并建议它有一个作用，在形成胚外组织，心脏和神经嵴衍生物。(C)出版社：Academic Press
We employed the yeast two-hybrid technique to screen a mouse embryo cDNA library for novel tissue-specific Class B basic helix-loop-helix (bHLH) transcription factors, which heterodimerize with the ubiquitously expressed Class A bHLH protein E12. From this screen, we cloned a novel bHLH protein, which we named eHAND. Its low sequence identity with other bHLH family members and unique expression pattern during development suggest that eHAND defines a new subclass of Class B bHLH proteins. eHAND was expressed at high levels in trophoblast cells and extraembryonic membranes throughout development. The first site of eHAND expression in embryos was the heart, where it was expressed at high levels between 8.5 and 10.5 days post coitum (d.p.c.), after which transcript levels declined abruptly. By 13.5 d.p.c., eHAND expression in the heart was localized to regions of valve formation, Expression in other regions of the embryo was confined to tissues with a substantial neural crest component, eHAND was expressed in the first branchial arch and its derivatives, in the sympathoadrenal lineage, and in the enteric system. The expression pattern of eHAND during development is distinct from that of other bHLH genes and suggests that it has a role in formation of extraembryonic tissues, heart, and neural crest derivatives. (C) 1995 Academic Press, Inc.