The specific direct interaction of helper T cells and antigen-presenting B cells. II. Reorientation of the microtubule organizing center and reorganization of the membrane-associated cytoskeleton inside the bound helper T cells.
The specific direct interaction of helper T cells and antigen-presenting B cells. II. Reorientation of the microtubule organizing center and reorganization of the membrane-associated cytoskeleton inside the bound helper T cells.
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DOI：
10.1084/jem.165.6.1565
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发表时间：
1987-06-01
期刊：
影响因子：
--
通讯作者：
Singer SJ
中科院分区：
文献类型：
--
作者：
Kupfer A;Swain SL;Singer SJ
我们已经产生并研究了克隆的Th细胞或T杂交瘤细胞与ag -present B杂交瘤细胞或B淋巴瘤细胞之间形成的细胞偶。结合的Th细胞内发生的两次重排证明了Th和B-APC之间的直接相互作用；MTOC（可能还有GA）朝向与B细胞接触的细胞区域，并且一种与膜相关的细胞骨架蛋白talin在接触的Th膜下集中。在缺乏特异的Ag或正确的Ia决定因子的情况下，在光镜下形成的非特异性T- b细胞偶对在形态上与特异的细胞偶对难以区分，但在这种非特异性偶对结合的T细胞内既不发生MTOC也不发生talin重排。此外，在特定的T-B偶对中，需要B细胞加工Ag来产生MTOC和talin重排。在同种异体Th- B细胞偶对的情况下，在Th细胞内观察到类似的特异性MTOC和talin重排。细胞外Ca2+是MTOC定向发生在特异性结合的Th细胞内所必需的，但不是talin重排所必需的。我们提出MTOC（和GA）重定向和talin重排参与GA衍生淋巴因子从Th细胞定向分泌到结合的B细胞。
We have produced and investigated cell couples formed between cloned Th cells or T hybridoma cells, and either Ag-presenting B hybridoma or B lymphoma cells. The specific direct interaction between a Th and B-APC is here demonstrated by two rearrangements occurring inside the bound Th cell; the MTOC (and presumably the GA) is oriented to face the cell contact region with the B cell, and a membrane-associated cytoskeletal protein, talin, becomes concentrated under the contacting Th membrane. In the absence of the specific Ag or the correct Ia determinant, nonspecific T-B cell couples form that are morphologically indistinguishable from specific cell couples in the light microscope, but neither the MTOC nor the talin rearrangement occurs inside the bound T cell of such nonspecific couples. Furthermore, Ag processing by the B cell is required to produce the MTOC and talin rearrangements within the T cell in specific T-B couples. In the case of allogeneic Th- B cell couples, similar specific MTOC and talin rearrangements are observed inside the Th. Extracellular Ca2+ is required for the MTOC orientation to occur inside the specifically bound Th cell, but not for the talin rearrangement. It is proposed that the MTOC (and GA) reorientation and the talin rearrangement are involved in the directed secretion of GA-derived lymphokines from the Th cell to the bound B cell.