Endosomal proteolysis of internalized proteins
Endosomal proteolysis of internalized proteins
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DOI：
10.1016/0014-5793(96)00368-7
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发表时间：
1996-06-24
期刊：
影响因子：
3.5
通讯作者：
Bergeron, JJM
中科院分区：
文献类型：
--
作者：
Authier, F;Posner, BI;Bergeron, JJM
内体蛋白水解酶与内化调节多肽的降解有关，内化调节多肽参与代谢途径的控制和细胞内抗原的处理，以产生细胞溶解免疫反应。由于依赖于ATP的质子泵的存在，调节蛋白酶活性、蛋白质展开和受体-配体相互作用的依赖于ATP的质子泵的存在，调节内吞体囊泡中的加工过程。与溶酶体相比，有限数量的蛋白酶似乎驻留在不包含能够完全降解所有内化的多肽的活性蛋白酶的完整补充中，一些酸性水解酶显然与它们与未确定的内吞体膜受体的结合有关，酶的数量有限以及内酶体内从中性到酸性(pH 7至5)的pH梯度使与溶酶体相比具有选择性和可控的加工环境成为可能。将蛋白质分解成氨基酸的全套内切和外切酶在溶酶体的途径中后期活跃。
Endosomal proteases have been implicated in the degradation of internalized regulatory peptides involved in the control of metabolic pathways and in the processing of intracellular antigens for cytolytic immune responses. Processing in the endocytic vesicles is regulated by changes in endosomal acidity due to the presence of an ATP-dependent proton pump which modulates protease activity, protein unfolding and receptor-ligand interactions, A limited number of proteases appear to reside in endosomes which do not contain the full complement of active proteases capable of completely degrading all internalized polypeptides, Retention of some acid hydrolases in endosomes is apparently related to their association with undefined endosomal membrane receptors, The limited number of proteases and the pH gradient from neutral to acidic (pH 7 to 5) within endosomes make possible a selective and controlled processing environment in comparison to lysosomes, The full set of endo- and exopeptidases that break down proteins to amino acids are active later in the pathway in lysosomes.