A novel rat tetraspan protein in cells of the oligodendrocyte lineage
A novel rat tetraspan protein in cells of the oligodendrocyte lineage
复制标题
DOI：
10.1046/j.1471-4159.1999.0732600.x
复制
发表时间：
1999-12-01
影响因子：
4.7
通讯作者：
Brophy, PJ
中科院分区：
文献类型：
--
作者：
Birling, MC;Tait, S;Brophy, PJ
四跨膜蛋白/跨膜4超家族基因超家族编码跨越质膜四次的蛋白质。Tetraspan蛋白参与各种细胞类型的增殖、运动和分化，并且在某些细胞中它们可以将质膜蛋白连接成信号复合物。使用从少突胶质细胞及其祖细胞制备的消减cDNA文库，我们已经确定Tspan-2为该超家族的成员。原位杂交分析显示，与Plp基因（CNS中髓鞘形成神经胶质细胞的一种特征性标记物）相比，少突胶质细胞谱系的细胞中有稳健的表达。大鼠Tspan-2 mRNA仅限于神经系统，出生后不久在CNS中可通过北方印迹检测到。随后，该基因在出生后第3天至第10天之间强烈上调，表达水平持续升高至出生后第22天。这些数据表明，Tspan-2是可能发挥作用的信号在少突胶质细胞在其终末分化成髓鞘形成神经胶质细胞的早期阶段，也可能在稳定成熟鞘的功能。
The tetraspanin/transmembrane 4 superfamily gene superfamily encodes proteins that span the plasma membrane four times. Tetraspan proteins are implicated in proliferation, motility, and differentiation in various cell types, and in some cells they may link plasma membrane proteins into signalling complexes, Using a subtractive cDNA library prepared from oligodendrocytes and their progenitor cells, we have identified Tspan-2 as a member of this superfamily. In situ hybridization analysis revealed robust expression in cells of the oligodendrocyte lineage in comparison with the Plp gene, a well-characterized marker for myelin-forming glia in the CNS. Rat Tspan-2 mRNA is restricted to the nervous system and is detectable by northern blot shortly after birth in the CNS, Subsequently the gene is up-regulated strongly between postnatal day 3 and 10, and expression levels continue to rise up to postnatal day 22. These data indicate that Tspan-2 is likely to play a role in signalling in oligodendrocytes in the early stages of their terminal differentiation into myelin-forming glia and may also function in stabilizing the mature sheath.