The relationship between cell membrane damage and lipid peroxidation under the condition of hypoxia‐reoxygenation: analysis of the mechanism using antioxidants and electron transport inhibitors
The relationship between cell membrane damage and lipid peroxidation under the condition of hypoxia‐reoxygenation: analysis of the mechanism using antioxidants and electron transport inhibitors
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DOI：
10.1002/cbf.1578
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发表时间：
2009-08
影响因子：
3.6
通讯作者：
D. Yajima;Hisako Motani;Mutsumi Hayakawa;Yayoi Sato;Kaoru Sato;H. Iwase
中科院分区：
文献类型：
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作者：
D. Yajima;Hisako Motani;Mutsumi Hayakawa;Yayoi Sato;Kaoru Sato;H. Iwase
我们连续观察了缺氧-复氧（H/R）条件下细胞的脂质过氧化和细胞膜损伤，并通过使用电子传递抑制剂和抗氧化剂分析了其机制。在H/R实验中，在缺氧阶段观察到脂质过氧化和细胞膜损伤。在复氧阶段，脂质过氧化反应停止，而细胞膜损伤没有。抗氧化剂，N-乙酰半胱氨酸（NAC）和氰化钾（KCN）抑制脂质过氧化和细胞膜损伤，而鱼藤酮不抑制它们中的任何一个。虽然抗霉素A不抑制脂质过氧化，但它在缺氧阶段抑制细胞膜损伤，但在复氧阶段不抑制。这些结果表明，脂质过氧化可以影响细胞膜损伤作为一个触发器在缺氧阶段和氧化应激的产生可以根据电子传递系统中的抑制位置而有所不同。版权所有© 2009约翰威利父子有限公司。
We consecutively observed lipid peroxidation and cell membrane damage under the condition of hypoxia‐reoxygenation (H/R) in cells and analyzed their mechanisms by using electron transport inhibitors and an antioxidant. In H/R experiments, lipid peroxidation and cell membrane damage were observed during the hypoxia phase. In the reoxygenation phase, lipid peroxidation stopped, while cell membrane damage did not. An antioxidant, n‐acetylcystein (NAC), and potassium cyanide (KCN) inhibited lipid peroxidation and cell membrane damage, while rotenone did not inhibit either of them. Although antimycin A did not inhibit lipid peroxidation, it inhibited cell membrane damage during the hypoxia phase but not during the reoxygenation phase. These results suggested that lipid peroxidation can affect cell membrane damage as a trigger during the hypoxia phase and the generation of oxidative stress can vary depending on the inhibition locations in the electron transport system. Copyright © 2009 John Wiley & Sons, Ltd.