Pellicle complex ofEuglena gracilis: Characterization by disruptive treatments
Pellicle complex ofEuglena gracilis: Characterization by disruptive treatments
复制标题
纤细眼虫的表膜复合体：破坏性处理的表征
作者：
H. Silverman;R. S. Hikida
用4%秋水仙碱、0.5%β-巯基乙醇、0.5%Triton X-100和8%单宁酸处理纤细裸藻，试图对其膜层复合体进行表征。秋水仙碱对膜、管或水库的微管无明显影响。二甲基亚砜中的秋水仙碱使储藏区解体。秋水仙素是通过抑制鞭毛再生而进入细胞的。巯基乙醇破坏了细胞中的大部分细胞器。只有膜复合体的三部分质膜保持正常。细胞的微管和质膜下的蛋白质层被巯基乙醇处理破坏。Triton X-100使内细胞器膜变圆、向上膨胀和肿胀，但对质膜形态无影响。每一次治疗后，膜层的脊沟形态保持不变。巯基乙醇导致破裂的微管位置上的脊状凹陷，暗示微管的支持功能。Triton X-100对内质网的扩张更清楚地显示了它与膜复合体的联系。在一系列破坏性处理下，纤细眼球藻的细胞膜具有保持其完整性的能力。
SummaryEuglena gracilis was treated with 4% colchicine, 0.5% β-mercaptoethanol, 0.5% Triton X-100, and 8% tannic acid in attempting to characterize its pellicle complex. Colchicine had no visible effects on the microtubules of the pellicle, canal or reservoir. Colchicine in dimethyl sulfoxide disorganized the reservoir region. Colchicine was shown to enter the cell by its ability to inhibit flagellar regeneration. Mercaptoethanol destroyed most of the organelles in the cell. Only the tripartite plasmalemma of the pellicle complex remained normal. The microtubules of the cell and the protein layer underlying the plasmalemma were disrupted by mercaptoethanol treatment. Triton X-100 caused membranes of internal organelles to round, up become distended and swollen, but had no morphological effect on the plasmalemma. The ridge-groove shape of the pellicle remained intact after every treatment. Mercaptoethanol caused an indentation of the ridges over the position of a disrupted microtubule, suggesting a supporting function of the microtubules. Distension of endoplasmic reticulum by Triton X-100 revealed more clearly its association with the pellicle complex. The ability of the pellicle ofEuglena gracilis to maintain its integrity under a number of disruptive treatments was demonstrated.