Endurance training effects on neurotransmitter release in rat striatum: An in vivo microdialysis study
Endurance training effects on neurotransmitter release in rat striatum: An in vivo microdialysis study
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DOI：
10.1046/j.1365-201x.1997.00118.x
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发表时间：
1997-04-01
期刊：
影响因子：
--
通讯作者：
Michotte, Y
中科院分区：
文献类型：
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作者：
Meeusen, R;Smolders, I;Michotte, Y
在本研究中，我们使用在体微透析采样技术注册的训练和未经训练的大鼠纹状体细胞外的神经递质水平。我们进一步评估1小时的运动对纹状体释放多巴胺（DA），去甲肾上腺素（NA），谷氨酸（GLU）和γ-氨基丁酸（GABA）在训练和未经训练的大鼠的影响。男性维斯塔被随机分配到训练组或对照组。运动训练包括6周的跑台训练，每周5天，跑步时间和速度从第一周的30 min以19 m min（-1）逐渐增加到最后训练周的80 min以26 m min（-1）。对照组的动物每周两次置于跑步机上，总共接受四次“适应期”，在此期间，它们以26 m min（-1）的速度运动15-45 min。采用微孔液相色谱法（LC）、电化学检测（单胺和GABA）和荧光检测（GLU）分析脑透析液。比目鱼肌柠檬酸合成酶和纹状体DA、NA和GLU的基础浓度在训练组和对照组之间存在显著差异。60分钟的运动显着增加细胞外DA，NA和GLU水平在两组中，但没有统计学上的显着差异，在运动诱导的增加训练和对照动物。训练组和对照组之间的基础或运动诱导的GABA水平没有统计学差异。结果表明，运动训练似乎会导致纹状体神经递质的基础活动减少，同时保持对急性运动反应的必要敏感性。
In the present study we use the in vivo microdialysis sampling technique to register extracellular levels of neurotransmitters in the striatum of trained and untrained rats. We further evaluate the influence of 1 h of exercise on the striatal release of dopamine (DA), noradrenaline (NA), glutamate (GLU) and gamma-aminobutyric acid (GABA) in trained and untrained rats. Male Wistars were randomly assigned to a training or control group. The exercise training consisted of running on a treadmill for 6 weeks, 5 days week(-1), with running time and speed gradually increased from 30 min at 19 m min(-1) during the first week to 80 min at 26 m min(-1) during the final training week. The animals of the control group were placed on the treadmill twice a week, and received a total of four 'adaptation sessions', in which they exercised 15-45 min at 26 m min(-1). Brain dialysates were analysed with microbore liquid chromatography (LC), with electrochemical detection (monoamines and GABA) and fluorescence detection (GLU). Soleus citrate synthase and basal striatal concentrations of DA, NA and GLU were significantly different between the trained and control animals. Sixty minutes of exercise significantly increased extracellular DA, NA and GLU levels in both groups, but there was no statistically significant difference in the exercise-induced increase between trained and control animals. There was no statistical difference in basal or exercise-induced GABA levels between trained and control animals. The results indicate that exercise training appears to result in diminished basal activity of striatal neurotransmitters, while maintaining the necessary sensitivity for responses to acute exercise.