Spatiotemporal changes in environmental DNA concentrations caused by fish spawning activity.
Spatiotemporal changes in environmental DNA concentrations caused by fish spawning activity.
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鱼类产卵活动引起的环境 DNA 浓度的时空变化。
DOI：
10.1016/j.ecolind.2022.109213
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发表时间：
2022
影响因子：
6.9
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Wu;L.;Yamamoto;Y.;Yamaguchi;S.;Minamoto;T.
确定产卵的时间和地点对水生物种的保护和管理至关重要。环境DNA （Environmental DNA, eDNA）分析可通过检测eDNA浓度峰值和核DNA (nuDNA)/线粒体DNA （mtDNA）比值来监测鱼类产卵活动；然而，水体中产卵活动产生的高浓度eDNA的持续时间和扩散距离仍然未知，这使得我们无法制定准确有效的调查计划来监测产卵活动。本文以鲤鱼为研究对象，通过人工产卵实验，研究了鲤鱼产卵过程中eDNA浓度的时空变化。结果表明：产卵活动增加的nuDNA浓度、mtDNA浓度和nuDNA/mtDNA比值在达到峰值后每0.1 h分别以7.78%、5.22%和2.56%的速率下降；通过数据模拟，估计在不同时间和距离间隔的重复采样中成功监测鲤鱼产卵活动的概率。结果表明，通过eDNA分析可以监测24 h内鲤鱼的产卵活动。此外，当鲤鱼产卵活动发生一次时，根据每100米和每24小时采样一次的采样计划，通过测量nuDNA浓度或nuDNA/mtDNA比值，可以成功地监测产卵活动，概率约为50 - 75%。因此，利用eDNA分析可以对水生物种的产卵活动进行高时空精度的估计。
Determining the timing and location of spawning is critical for the conservation and management of aquatic species. Environmental DNA (eDNA) analysis can be used to monitor fish spawning activity by detecting peaks in eDNA concentrations and nuclear DNA (nuDNA)/mitochondrial DNA (mtDNA) ratios; however, the duration and diffusion distance of high concentrations of eDNA produced by spawning activities in water bodies are still unknown, preventing us from making accurate and effective survey plans to monitor spawning activities. Using common carp as a target species, we conducted artificial spawning experiments to investigate the spatiotemporal changes in eDNA concentrations during spawning. The results showed that the nuDNA concentration, mtDNA concentration, and nuDNA/mtDNA ratio that were increased by spawning activity decreased at rates of 7.78 %, 5.22 %, and 2.56 %, respectively, per 0.1 h after reaching the peak. Data simulations were performed to estimate the probability of successful monitoring of carp spawning activity during repeated sampling at different times and distance intervals. The results showed that carp spawning activity over the past 24 h could be monitored by eDNA analysis. Additionally, when carp spawning activity occurs once, spawning activity can be successfully monitored by measuring the nuDNA concentration or the nuDNA/mtDNA ratio with a probability of approximately 50–75 % based on a sampling plan of sample collection every 100 m and every 24 h. Thus, the spawning activity of aquatic species can be estimated with high spatial and temporal accuracy using eDNA analysis.