Xylem-specific gene expression in loblolly pine
Xylem-specific gene expression in loblolly pine
复制标题
火炬松木质部特异性基因表达
作者：
C. Loopstra;R. Sederoff
火炬松（Pinus taeda L.）从cDNA和基因组文库中克隆并命名为PtX 3 H6和PtX 14 A9。PtX 3 H6和PtX 14 A9的转录本在分化木质部中非常丰富，在针叶中不太丰富，在胚和巨配子体中非常低或不可检测。PtX 3 H6含有一个假定的信号肽，一个富含苏氨酸的区域，一个富含脯氨酸的区域，和一个疏水性的尾部。Pro-Pro-Pro-Val-X-X的重复序列与富含脯氨酸的细胞壁蛋白中发现的重复序列相似。PtX 3 H6和PtX 14 A9的氨基酸组成与阿拉伯半乳聚糖蛋白（AGPs）的氨基酸组成相似。PtX 14 A9含有与黑麦草、胡萝卜和玫瑰AGP的氨基末端序列相似的8个氨基酸序列。在这两个基因的5′侧翼区发现的7 bp序列先前已被证明参与GRP 1.8的血管特异性表达，GRP 1.8是在豆类中发现的富含甘氨酸的蛋白质。该序列也存在于另一种富含甘氨酸的豆类蛋白GRP 1.0的上游，并且可能部分负责pTx 3 H6和PtX 14 A9的木质部特异性表达。
Two genes preferentially expressed in differentiating xylem of loblolly pine (Pinus taeda L.) were cloned from cDNA and genomic libraries and designated PtX3H6 and PtX14A9. Transcripts of PtX3H6 and PtX14A9 are very abundant in differentiating xylem, less abundant in needles, and very low or nondetectable in embryos and megagametophytes. PtX3H6 contains a putative signal peptide, a threonine-rich region, a proline-rich region, and a hydrophobic tail. Repeats of Pro-Pro-Pro-Val-X-X are similar to repeats found in proline-rich cell wall proteins. The amino acid compositions of PtX3H6 and PtX14A9 are similar to those of arabinogalactan proteins (AGPs). PtX14A9 contains an 8 amino acid sequence similar to amino terminal sequences of ryegrass, carrot and rose AGPs.Upstream sequences have been determined from genomic clones encoding PtX3H6 and PtX14A9. A 7 bp sequence found in the 5′ flanking regions of both genes has previously been shown to be involved in the vascular-specific expression of GRP 1.8, a glycine-rich protein found in bean. The sequence is also present upstream of another glycine-rich protein from bean, GRP 1.0, and may be partially responsible for the xylem-specific expression of pTx3H6 and PtX14A9.
阿拉伯半乳聚糖-来自玫瑰细胞悬浮培养基和质膜的蛋白质。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Komalavilas,P;Zhu,JK;Nothnagel,EA
通讯作者：
Nothnagel,EA