PARTICIPATION OF THE PROTEIN G(O) IN MULTIPLE ASPECTS OF BEHAVIOR IN C-ELEGANS
PARTICIPATION OF THE PROTEIN G(O) IN MULTIPLE ASPECTS OF BEHAVIOR IN C-ELEGANS
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DOI：
10.1126/science.7886455
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发表时间：
1995-03-17
期刊：
影响因子：
56.9
通讯作者：
STERNBERG, PW
中科院分区：
文献类型：
--
作者：
MENDEL, JE;KORSWAGEN, HC;STERNBERG, PW
编码异源三聚体鸟苷三磷酸结合蛋白α亚基的果阿-1基因来自秀丽隐杆线虫的（G蛋白）G（o）在大多数神经元中表达，并且在参与产卵和雄性交配的肌肉中表达，果阿-1的功能减少突变引起多种行为缺陷，包括运动过度活跃、过早产卵和雄性阳痿，活化的G（o）α亚基的表达（G α（o））在转基因线虫中的诱导表达导致昏睡运动、延迟产卵和降低交配效率。在成虫中诱导表达活化的G α（o）足以引起这些表型，表明G α（o）通过其在神经元功能和专门肌肉功能中的作用来介导行为。
The goa-1 gene encoding the alpha subunit of the heterotrimeric guanosine triphosphate-binding protein (G protein) G(o) from Caenorhabditis elegans is expressed in most neurons, and in the muscles involved in egg laying and male mating, Reduction-of-function mutations in goa-1 caused a variety of behavioral defects including hyperactive movement, premature egg laying, and male impotence, Expression of the activated G(o) alpha subunit (G alpha(o)) in transgenic nematodes resulted in lethargic movement, delayed egg laying, and reduced mating efficiency, Induced expression of activated G alpha(o) in adults was sufficient to cause these phenotypes, indicating that G alpha(o) mediates behavior through its role in neuronal function and the functioning of specialized muscles.