Differential Changes in Neuronal Excitability in the Spinal Dorsal Horn After Spinal Nerve Ligation in Rats.
Differential Changes in Neuronal Excitability in the Spinal Dorsal Horn After Spinal Nerve Ligation in Rats.
复制标题
大鼠脊髓神经结扎后脊髓背角神经元兴奋性的差异变化。
DOI：
10.1007/s11064-016-2003-0
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Sugimoto T.
中科院分区：
文献类型：
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作者：
Terayama R;Yamamoto Y;Kishimoto N;Tabata M;Maruhama K;Iida S;Sugimoto T.
以往的研究表明，周围神经损伤引起脊髓背角过度的神经元反应和胶质细胞活化，这种变化已被提出来反映神经病理性疼痛状态的发展和维持。本研究的目的是研究周围神经损伤后脊髓背角神经元的兴奋性和胶质细胞的激活。我们研究了伤害性热刺激诱导的c-Fos蛋白样免疫反应性（Fos-LI）神经元在第四至第六腰椎（L4-L 6）水平脊髓背角神经元后，第五腰脊神经结扎（L5 SNL）。还在脊髓的组织切片中进行OX-42和GFAP的免疫荧光标记。SNL后3天，脊髓背角L4水平Fos-LI神经元的数量显著增加，但在损伤后14天恢复到与假手术对照组相似的水平。正如预期的那样，在SNL后3天发现L5水平脊髓背角中Fos-LI神经元分布的数量减少。然而，这些配置文件已再次出现在大量的14和21天后受伤。OX-42和GFAP的免疫荧光标记表明脊髓背角中的小胶质细胞和星形胶质细胞的顺序激活。我们的结论是，神经损伤导致脊髓背角神经元兴奋性的差异变化，这可能与胶质细胞激活。这些变化可能在周围神经损伤后神经病理性疼痛的发病机制中起重要作用。
Previous studies demonstrated that peripheral nerve injury induced excessive neuronal response and glial activation in the spinal cord dorsal horn, and such change has been proposed to reflect the development and maintenance of neuropathic pain states. The aim of this study was to examine neuronal excitability and glial activation in the spinal dorsal horn after peripheral nerve injury. We examined noxious heat stimulation-induced c-Fos protein-like immunoreactivity (Fos-LI) neuron profiles in fourth-to-sixth lumbar (L4–L6) level spinal dorsal horn neurons after fifth lumbar spinal nerve ligation (L5 SNL). Immunofluorescence labeling of OX-42 and GFAP was also performed in histological sections of the spinal cord. A significant increase in the number of Fos-LI neuron profiles in the spinal dorsal horn at the L4 level was found at 3 days after SNL, but returned to a level similar to that in sham-operated controls by 14 days after injury. As expected, a decrease in the number of Fos-LI neuron profiles in the spinal dorsal horn at the L5 level was found at 3 days after SNL. However, these profiles had reappeared in large numbers by 14 and 21 days after injury. Immunofluorescence labeling of OX-42 and GFAP indicated sequential activation of microglia and astrocytes in the spinal dorsal horn. We conclude that nerve injury causes differential changes in neuronal excitability in the spinal dorsal horn, which may coincide with glial activation. These changes may play a substantial role in the pathogenesis of neuropathic pain after peripheral nerve injury.