Homology in the transfer origins of broad host range IncP plasmids: definition of two subgroups of P plasmids.
Homology in the transfer origins of broad host range IncP plasmids: definition of two subgroups of P plasmids.
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广泛宿主范围 IncP 质粒转移起源的同源性：P 质粒两个亚组的定义。
DOI：
10.1007/bf00392187
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Guiney,G
中科院分区：
文献类型：
--
作者：
Yakobson,E;Guiney,G
广泛宿主范围的转移系统IncP质粒越来越多地被用于许多革兰氏阴性菌的遗传分析和操作。我们研究了属于IncP不亲和组的10个不同广宿主质粒转移起点的结构和功能相关性。这里报道的数据，加上我们关于这些质粒复制片段的相关性的结果，表明所有IncP质粒的基因组在指定转移起点和复制功能的区域具有广泛的序列同源性。同源性分析结果表明，INCP中存在两个亚类，分别命名为INCPα和INCPβ。此外，对IncP质粒转移起点的功能分析强烈表明，在接合过程中，DNA-缺口松弛复合体(Guiney和Helinski 1979)是质粒转移所必需的。
The transfer systems of broad host rangeIncP plasmids are increasingly used in the genetic analysis and manipulation of many gram-negative bacteria. We have examined the structural and functional relatedness of the transfer origins of ten different broad host range plasmids which belong to the IncP incompatibility group. The data reported here, together with our results on relatedness of the replication segments of these plasmids, demonstrate that the genomes of all IncP plasmids share extensive sequence homology in the regions specifying the transfer origin and replication functions. The homology results reveal the existence of two subclasses among IncP plasmids, designated here as IncPα and IncPβ. Furthermore, a functional analysis of the transfer origins of IncP plasmids suggests strongly that the DNA-nicking relaxation complex (Guiney and Helinski 1979) is required for plasmid transfer during conjugation.