MYASTHENIA-GRAVIS - QUANTITATIVE IMMUNOCYTOCHEMICAL ANALYSIS OF INFLAMMATORY CELLS AND DETECTION OF COMPLEMENT MEMBRANE ATTACK COMPLEX AT THE END-PLATE IN 30 PATIENTS
MYASTHENIA-GRAVIS - QUANTITATIVE IMMUNOCYTOCHEMICAL ANALYSIS OF INFLAMMATORY CELLS AND DETECTION OF COMPLEMENT MEMBRANE ATTACK COMPLEX AT THE END-PLATE IN 30 PATIENTS
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DOI：
10.1212/wnl.43.6.1167
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发表时间：
1993-06-01
期刊：
影响因子：
9.9
通讯作者：
ENGEL, AG
中科院分区：
文献类型：
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作者：
NAKANO, S;ENGEL, AG
为探讨细胞免疫效应与体液免疫效应在重症肌无力（MG）中的作用，我们检测了30例MG患者肌肉中炎性细胞的出现情况，确定了MG患者肌肉中炎性细胞的聚集部位，（在终板处或远离终板），对终板处的那些细胞进行计数和免疫表型分析，并评估补体膜攻击复合物（MAC）在终板处的沉积频率。30例患者中有7例在肌肉中有明确的单核细胞聚集（淋巴出血），但这些与终板无关。30例患者中有20例在终板处或附近有炎性细胞（主要是巨噬细胞和T细胞），但这些细胞存在于不到10%的终板中，并且很少在任何终板上有一个以上的细胞。无炎性细胞穿透终板附近的肌纤维。所有患者的所有终板均进行MAC免疫染色。研究结果再次证实，MG的主要免疫效应反应是体液介导的。肌肉和其他组织中的淋巴出血可能是MG免疫调节紊乱的非特异性指标。
To clarify the role of cell-mediated versus humoral immune effector responses in myasthenia gravis (MG), we examined the occurrence of inflammatory cells in muscle from 30 patients with MG, determined the site of accumulation of the cells (at or remote from end-plates), enumerated and immunophenotyped those cells at the end-plate, and evaluated the frequency of deposition of the complement membrane attack complex (MAC) at the end-plate. Seven of 30 patients had well-defined collections of mononuclear cells in muscle (lymphorrhages), but these were not topographically related to the end-plates. Twenty of 30 patients had inflammatory cells (mostly macrophages and T cells) at or near end-plates, but these cells were present at less than 10% of the end-plates, and only seldom was there more than one cell at any end-plate. None of the inflammatory cells penetrated the muscle fiber near the end-plate. All end-plates of all patients immunostained for MAC. The findings reconfirm that the predominant immune effector response in MG is humorally mediated. The lymphorrhages in muscle and other tissues are probably a nonspecific indicator of disturbed immune regulation in MG.