Unexpected finding of Fusobacterium varium as the dominant Fusobacterium species in cattle rumen: potential implications for liver abscess etiology and interventions
Unexpected finding of Fusobacterium varium as the dominant Fusobacterium species in cattle rumen: potential implications for liver abscess etiology and interventions
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意外发现变异梭杆菌作为牛瘤胃中的优势梭杆菌：对肝脓肿病因和干预措施的潜在影响
DOI：
10.1093/jas/skad130
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发表时间：
2023
影响因子：
3.3
通讯作者：
Alvarez, Pedro J. J.
中科院分区：
文献类型：
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作者：
Schwarz, Cory;Mathieu, Jacques;Gomez, Jenny Laverde;Miller, Megan R.;Tikhonova, Marina;Nagaraja, Tiruvoor. G.;Alvarez, Pedro J. J.
在牛的瘤胃微生物组研究中，变异梭杆菌通常被忽视，而可能更多的是引起肝脓肿的坏死梭杆菌。然而，在牛的瘤胃液中和在为富含F而量身定做的培养条件下，发现了富含F。尸检。使用近全长的16S核糖体核糖核酸测序，我们证明了f。变量在通常用于枚举F的限制条件下生长。尸斑，表明之前的F。尸检丰度评估可能是不准确的，而且。变种可能是瘤胃细菌群落中被低估的成员。变种梭杆菌对ASF不那么敏感。饲料中的尸磷抗生素通常用于饲养场。泰乐菌素是目前用于减少牛肝脓肿策略的黄金标准，暴露于泰乐菌素一直阻碍F。与未暴露的对照相比，尸检菌株的检测结果超过67%(P<； 0.05)。与之相反，杂色镰刀菌完全抗性或高度抗性(最高产量下降0%-13%，P<； 0.05)。莫能菌素是一种离子载体抗生素，对F有较强的抑制活性。尸检结果：F。Varium。最后，对两个F.从瘤胃分离的不同菌株揭示了与致病性人F相关的毒力基因的存在。与哺乳动物细胞主动入侵相关的各种分离株。这里提供的数据鼓励对生态作用的进一步调查。牛瘤胃内的各种细菌和在肝脓肿发展中的潜在作用，以及积极的干预措施。
Fusobacterium variumhas been generally overlooked in cattle rumen microbiome studies relative to the presumably more abundant liver abscess-causingFusobacterium necrophorum. However,F. variumwas found to be more abundant in the rumen fluid of cattle and under culture conditions tailored to enrichF. necrophorum. Using near-full length 16S ribosomal ribonucleic acid sequencing, we demonstrate thatF. variumgrows under restrictive conditions commonly used to enumerateF. necrophorum, suggesting that previousF. necrophorumabundance assessment may have been inaccurate and thatF. variummay be an underestimated member of the ruminal bacterial community.Fusobacterium variumwere not as susceptible asF. necrophorumto in-feed antibiotics conventionally used in feedlots. Exposure to tylosin, the current gold standard for liver abscess reduction strategies in cattle, consistently hindered growth of theF. necrophorumstrains tested by over 67% (P< 0.05) relative to the unexposed control. In contrast,F. variumstrains were totally or highly resistant (0%–13% reduction in maximum yield,P< 0.05). Monensin, an ionophore antibiotic, had greater inhibitory activity againstF. necrophorumthanF. varium. Finally, preliminary genomic analysis of twoF. variumisolates from the rumen revealed the presence of virulence genes related to those of pathogenic humanF. variumisolates associated with active invasion of mammalian cells. The data presented here encourage further investigation into the ecological role ofF. variumwithin the bovine rumen and potential role in liver abscess development, and proactive interventions.