Biotransformation of ferulic acid to 4-vinylguaiacol by a wild and a diploid strain of Aspergillus niger
Biotransformation of ferulic acid to 4-vinylguaiacol by a wild and a diploid strain of Aspergillus niger
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DOI：
10.1016/j.biortech.2010.01.086
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发表时间：
2010-06-01
影响因子：
11.4
通讯作者：
Saucedo-Castaneda, Gerardo
中科院分区：
文献类型：
--
作者：
Baqueiro-Pena, Itzamna;Rodriguez-Serrano, Gabriela;Saucedo-Castaneda, Gerardo
阿魏酸的生物转化有许多有趣的工业用途。研究了野生菌株黑曲霉C28825和二倍体菌株DAR2对阿魏酸的生物转化。野生菌株C28825能将阿魏酸转化为香草酸(VA)，而二倍体菌株DAR2优先将阿魏酸脱羧为4-乙烯基愈创木酚(4VG)。后者用质谱仪、H-1和C-13核磁共振波谱进行了鉴定，并用高效液相色谱法进行了定量。二倍体菌株黑曲霉DAR2的阿魏酸转化率为%，野生菌株为36%。摩尔产量表明，在二倍体菌株培养中，4VG的形成是首选的，比VA的形成高4.4倍。参与阿魏酸生物转化的酶的不同调控可能解释了二倍体DAR2积累4VG的原因。该菌株同时产生4VG和VA。(C)2010爱思唯尔有限公司。保留所有权利。
Ferulic acid biotransformation has a number of interesting industrial uses. Ferulic acid biotransformation by the wild strain Aspergillus niger C28825 and a diploid strain DAR2, obtained by parasexual recombination, was studied. The wild strain of A. niger C28825 biotransforms ferulic acid to vanillic acid (VA): while the diploid strain DAR2 preferentially decarboxylates ferulic acid to 4-vinylguaiacol (4VG). The latter was identified by mass spectroscopy, H-1 and C-13 nuclear magnetic resonance spectroscopy, and quantified by HPLC. The diploid strain A. niger DAR2 and the wild strain showed a ferulic acid conversion of 64% and 36%, respectively. Molar yields show that the formation of 4VG was preferred, being as much as 4.4 times higher than the formation of VA in diploid strain cultures. Differential regulation of enzymes involved in the biotransformation of ferulic acid may explain the accumulation of 4VG by diploid DAR2. This strain produced both 4VG and VA. (C) 2010 Elsevier Ltd. All rights reserved.