Measuring multiple fluxes through plant metabolic networks
Measuring multiple fluxes through plant metabolic networks
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DOI：
10.1111/j.1365-313x.2005.02649.x
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发表时间：
2006-02-01
期刊：
影响因子：
7.2
通讯作者：
Shachar-Hill, Y
中科院分区：
文献类型：
--
作者：
Ratcliffe, RG;Shachar-Hill, Y
通过代谢网络的通量对细胞功能至关重要，了解这些通量对于理解和操纵代谢表型至关重要。标记提供了通量测量的关键，并且在网络通量分析中，多个通量的测量允许将通量图叠加在代谢网络上。两个互补的方法，动态和稳态标记的原理和实践，进行了描述，强调最佳实践，并说明其贡献的网络通量分析与植物和微生物文献中的例子。还描述了用于检测稳定同位素的主要分析方法，以及从标记数据获得通量图的程序。为方便起见，提供了一系列概括网络流量分析关键概念的方框。
Fluxes through metabolic networks are crucial for cell function, and a knowledge of these fluxes is essential for understanding and manipulating metabolic phenotypes. Labeling provides the key to flux measurement, and in network flux analysis the measurement of multiple fluxes allows a flux map to be superimposed on the metabolic network. The principles and practice of two complementary methods, dynamic and steady-state labeling, are described, emphasizing best practice and illustrating their contribution to network flux analysis with examples taken from the plant and microbial literature. The principal analytical methods for the detection of stable isotopes are also described, as well as the procedures for obtaining flux maps from labeling data. A series of boxes summarizing the key concepts of network flux analysis is provided for convenience.