A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients
A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients
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DOI：
10.1016/j.chroma.2014.10.094
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发表时间：
2014-12-12
影响因子：
4.1
通讯作者：
Liu, Huwei
中科院分区：
文献类型：
--
作者：
Li, Min;Tong, Xunliang;Liu, Huwei
将正相和反相液相色谱与四极杆飞行时间质谱联用，建立了一种不间断流动的在线二维液相色谱（2D LC）血脂分析方法，并将其应用于血浆中脂质的分离和鉴定，充分发挥了该方法在血脂定性和定量检测方面的优势。本文测定了人血浆中17类540种内源性脂质，并详细探讨了动脉粥样硬化患者和对照者血浆中脂质代谢产物的差异。19个验证标准品的检出限（LOD）均达到ng/mL量级，峰面积和保留时间的RSD分别为0.4-8.0%和0.010- 0.47%。此外，一对异构体，半乳糖神经酰胺（GalC）和葡萄糖神经酰胺（GluC），被成功地分离，表明只有GalC在动脉粥样硬化患者的水平显着增加，而不是GluC，与对照组相比（对照组与患者：比率为1.5-2.8倍增加）。这将有助于动脉粥样硬化的进一步研究。(C)2014爱思唯尔有限公司版权所有。
A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and reversed-phase LC with quadrupole time-of-flight mass spectrometry (QToF-MS), which was then applied to separate and identify the lipid species in plasma, making its merits in quality and quantity of the detection of lipids. Total 540 endogenous lipid species from 17 classes were determined in human plasma, and the differences in lipid metabolism products in human plasma between atherosclerosis patients and control subjects were explored in detail. The limit of detections (LODs) of 19 validation standards could all reach ng/mL magnitude, and the RSDs of peak area and retention time ranged 0.4-8.0% and 0.010-0.47%, respectively. In addition, a pair of isomers, galactosylceramides (GalC) and glucosylceramides (GluC), was successfully separated, showing that only the levels of GalC in atherosclerosis patients were significantly increasing, rather than GluC, compared with the controls (controls vs. patients: the ratio was 1.5-2.8-fold increasing). It would be helpful to the further research of the atherosclerosis. (C) 2014 Elsevier B.V. All rights reserved.